Companies / Amerigo Scientific / "10x Ammonium Buffer, 15 mM MgCl2"
Amerigo Scientific

"10x Ammonium Buffer, 15 mM MgCl2" | Amerigo Scientific

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures./

FEATURES

Promotes amplification on most DNA templates

High yield

High specificity

Tolerance for primer annealing temperature

10x Buffer/

AVAILABLE IN 4 FORMULATIONS:

15 mM MgCl2,

Mg2+ free

Tween free

Mg2+ free + Tween free/

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences. /

TIP - CHOOSE THE RIGHT BUFFER

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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