This multiplex ELISA kit for M1/M2/MDSC cytokines is designed for semi-quantitative and simultaneous determination of cytokines relevant to the proliferation of Myeloid Derived Suppressor Cells (MDSCs) and their differentiation toward M1 or M2 phenotype. The kit simultaneously determines granulocyte macrophage colony stimulating factor (GM-CSF), interferon-__(IFN-__interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-12 (IL-12), monocyte chemotactic and activating factor (MCAF, also known as MCP-1), and tumor necrosis factor-_ (TNF-_ in cell culture supernatant and other biological samples. In combination with other Anogen quantitative cytokine ELISA kits, the M1/M2/MDSC cytokine multiplex ELISA kit is expected to be useful for the investigation of the relationship of cytokine expression and MDSC induced inmmunosuppression in various disease models.