dsDNA Screen ELISA is a solid phase enzyme immunoassay with human recombinant double-stranded DNA (dsDNA) for the combined quantitative and qualitative detection of IgG, IgA and IgM antibodies against dsDNA in human serum. Anti-dsDNA antibodies mainly recognize the phosphate units of the DNA, thus these autoantibodies also bind single stranded DNA (ssDNA). To ensure correct quantitation of anti-dsDNA antibodies the used antigen has been proven to be free of contamination with ssDNA.
The assay is a tool in the differential diagnosis of systemic lupus erythematosus (SLE).