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eBook

SEP 19, 2025 6:46 AM PDT

One-Step Genotyping by Sequencing: A Guide to Faster Results

SPONSORED BY: LGC Biosearch Technologies

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Tired of multi-stage genotyping workflows that slow down your sequencing projects? This high-throughput, one-step genotyping by sequencing method delivers accurate, reproducible data across species and input levels, in less time and with fewer steps.

With room-temperature-stable reagents and automation-ready chemistry, this approach minimizes preparation time, reduces costs, and keeps your data quality high. Whether working in crop genotyping, polyploid wheat, or high-density maize, you’ll move from sample to results faster while maintaining consistency across marker types and ploidy levels.

In head-to-head trials with the original Amp-Seq method, the streamlined workflow delivered measurable gains across diverse DNA marker types and ploidy levels.

This free application note includes complete datasets demonstrating performance in:

Improved read mapping & on-target percentages - more usable sequencing data
Higher genotyping call rates & uniformity - consistent, reliable results
Low input DNA compatibility - ideal for limited or challenging samples
Cross-species flexibility - from wheat to maize and beyond

If your goal is to scale genotyping efficiency while maintaining the highest standards for sequencing accuracy, the data in this resource will help you choose the right approach for your research.

Download now to see how a single-step workflow can transform your sequencing projects.

About the Sponsor

LGC Biosearch Technologies

Biosearch Technologies is the comprehensive genomics portfolio from LGC, providing products and services for genomic analysis that support mission critical applications for global customers in agrigenomics and human healthcare.

The portfolio offers integrated tools and technologies to accelerate sample preparation, amplification, cloning and expression, next generation sequencing (NGS), custom oligonucleotide synthesis, nucleic acid chemistry reagents, and oligo therapeutics.

Whether through sbeadex™ DNA purification, oligos and reagents (KASP™, BHQplus™ Probes, BHQ™ Probe Master Mix), or proprietary sample prep and PCR instrumentation (oKtopure™, SNPline™, Nexar™ and IntelliQube™), we enable our customers from discovery to production-scale testing.

Biosearch Technologies is a leader in the agricultural genomics market and is increasingly adopted by researchers, with over 2,000 scientific papers citing KASP assays. Key applications include validation and screening of newly discovered SNPs in Marker-Assisted Selection and Marker-Assisted Breeding programmes for both plants and livestock.

Globally, our cGMP manufacturing and ISO 13485-compliant facilities support supply chain continuity, complementing our customers' product development pipelines through our robust research grade and cGMP oligonucleotide manufacturing that achieves consistent scale up. For molecular diagnostics, Biosearch Technologies has proven expertise in NGS, isothermal, qPCR-based applications where our tools and technologies such as BHQ probes and proprietary PCR platforms are frequently used in LDTs, ASRs and in vitro diagnostics. Through our recently acquired Lucigen portfolio, we also offer custom and bulk enzymes and buffers as critical components for molecular diagnostics.

Sales offices are located in China, Germany, Singapore, UK, and US. Sales representatives are available throughout Europe, Latin America, and Asia Pacific, with distributors in other key territories. Visit www.biosearchtech.com