50X Tris-Acetate EDTA (TAE)/
Used as a running buffer in PAGE and Agarose electrophoresis. Can also be used for in gel electrophoresis/
Typically used for the separation of nucleic acids DNA and RNA./
It is made up of Tris-acetate buffer, usually at pH 8.0, and EDTA, which sequesters divalent cations./
TAE has a lower buffer capacity than TBE/
Supplied as a 50X concentrate dilute to working strength./
Contents per litre (1x working solution)/
Tris acetate 0.04M
EDTA 0.001M
pH 8.0
DNase, RNase none detected