Automating CAR-T Flow Cytometry: From Complex Manual Prep to Standardized Automation with Pluto C-FREE™

CAR-T translational research demands precise, reproducible, and scalable flow cytometry workflows. Manual centrifugation and antibody preparation steps remain major sources of variability and cell loss. In this joint session, Dr. Raj Kurupati (Janssen) and Dr. Sashi (Curiox Biosystems) discuss practical strategies to overcome these challenges through automation using the Pluto C-FREE™ platform..

Raj shares Janssen’s experience scaling high-dimensional panels—achieving consistent results across 41+ color assays—while Sashi presents Curiox’s cross-site reproducibility data aligned with multi-lab standardization efforts. Together, they illustrate how automation transforms complex immunophenotyping into standardized, reproducible, and compliance-ready workflows for next-generation CAR-T programs.

Learning Objectives:

• Understand how automation supports high-parameter assay scalability in translational and CAR-T research without increasing workflow complexity.

• Learn practical approaches to automating antibody cocktailing and sample prep for flow cytometry. 

• Recognize how standardized, wash-free workflows improve cross-lab reproducibility and align with NIST reproducibility initiatives.

• Evaluate how accessible automation tools can meet regulatory expectations (e.g., 21 CFR Part 11 compliance) while enabling consistent, auditable processes in pharma environments.