Companies / Amerigo Scientific / ZediXcite Fluorogenic TG2-Assay Kit
Amerigo Scientific

ZediXcite Fluorogenic TG2-Assay Kit | Amerigo Scientific

Assay Principle

TISSUE TRANSGLUTAMINASE (TG2) cleaves a dark quenching molecule from the side chain of TG2 specific peptide A163 incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored (excitation wavelength 330 nm; emission wavelength 418 nm)./

Reagents in the kit

(1) SUBSTRATE REAGENT (SR): 2 x 23 μL TG2 specific peptide A163 (DMSO solution).

(2) BUFFER REAGENT (BR): 2 x 18 mL TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, glycine methyl ester (lyophilizate).

(3) DILUTION BUFFER (DB): 1 x 18 mL TRIS buffer pH 8.1 containing sodium chloride, EDTA, DTT (lyophilizate)./

Equipment

The ZediXcite Fluorogenic TG2-Assay Kit (F014) can be used in fluorescence microplate readers using microplates as well as in standard fluorescence spectrophotometers with cuvettes. Refer to the instructions of the manufacturer.

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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