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    Nucleic Acid Quantitation

    Nucleic Acid Quantitation: In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, along with their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic acids (such as DNA or RNA) in a solution. These are spectrophotometric quantification and UV fluorescence tagging in presence of a DNA dye.

    What's Popular in Nucleic Acid Quantitation

    Trending News

    • JUL 14, 2022
      Clinical & Molecular DX
      A magnetic bead for every need: Expanding applications with conjugated magnetic beads
      A magnetic bead for every need: Expanding applications with conjugated magnetic beads
      Magnetic beads (or superparamagnetic particles) can be used for the isolation and purification of cells, nucleic acids, ...
      Sponsored By: Cytiva
      6
      DEC 03, 2020
      Cell & Molecular Biology
      7 Considerations When Purchasing a Microvolume UV-Vis Spectrophotometer
      7 Considerations When Purchasing a Microvolume UV-Vis Spectrophotometer
      It all started with the Thermo Scientific™ NanoDrop™ 1000 as the first microvolume UV-Vis spectrophotometer, ...
      Sponsored By: Thermo Fisher Scientific
      1
      OCT 19, 2020
      Clinical & Molecular DX
      Making Capillary Electrophoresis Accessible for Any Lab
      Making Capillary Electrophoresis Accessible for Any Lab
      Analyzing nucleic acids through gel electrophoresis has been a staple of genetic research for decades. But using traditi ...
      Sponsored By: Promega
      2
      NOV 01, 2019
      Clinical & Molecular DX
      myTAIHEART Blood Test and Biopsy of Heart Transplant Recipients
      myTAIHEART Blood Test and Biopsy of Heart Transplant Recipients
      Transplant rejection is a process where the immune system of the patient consider the donor's organ as foreign ...
      Written By: Nupur Srivastava
      2
    JUL 14, 2022
    Clinical & Molecular DX
    A magnetic bead for every need: Expanding applications with conjugated magnetic beads
    A magnetic bead for every need: Expanding applications with conjugated magnetic beads
    Magnetic beads (or superparamagnetic particles) can be used for the isolation and purification of cells, nucleic acids, ...
    Sponsored By: Cytiva
    6
    DEC 03, 2020
    Cell & Molecular Biology
    7 Considerations When Purchasing a Microvolume UV-Vis Spectrophotometer
    7 Considerations When Purchasing a Microvolume UV-Vis Spectrophotometer
    It all started with the Thermo Scientific™ NanoDrop™ 1000 as the first microvolume UV-Vis spectrophotometer, ...
    Sponsored By: Thermo Fisher Scientific
    1
    OCT 19, 2020
    Clinical & Molecular DX
    Making Capillary Electrophoresis Accessible for Any Lab
    Making Capillary Electrophoresis Accessible for Any Lab
    Analyzing nucleic acids through gel electrophoresis has been a staple of genetic research for decades. But using traditi ...
    Sponsored By: Promega
    2
     
     
    NOV 01, 2019
    Clinical & Molecular DX
    myTAIHEART Blood Test and Biopsy of Heart Transplant Recipients
    myTAIHEART Blood Test and Biopsy of Heart Transplant Recipients
    Transplant rejection is a process where the immune system of the patient consider the donor's organ as foreign ...
    Written By: Nupur Srivastava
    2
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    Nucleic Acid Quantitation
    Trending News

    Webinars

    • MAR 20, 2024 | 8:00 AM
      Introducing the first dual reporter application for Luminex xMAP INTELLIFLEX DR-SE Instruments:  measurement of phosphorylated and total proteins on the same bead
      Introducing the first dual reporter application for Luminex xMAP...
      Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...
      Speaker: Sherry Dunbar, Ph.D., MBA , Sigrun Badrnya, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      11
      DEC 12, 2023 | 10:00 AM
      Characterization of LNPs via Density Matching AUC
      Characterization of LNPs via Density Matching AUC
      Determining the purity of LNPs loaded with nucleic acids can be challenging due to their size heterogeneity. Here, we present analytical ultracentrifugation (AUC) methods that use multiwavel...
      Speaker: Amy Henrickson
      Sponsored By: Beckman Coulter Life Sciences
      11
      JUN 08, 2023 | 10:40 AM
      Analytical Validation for qPCR with Live Q&A
      Analytical Validation for qPCR with Live Q&A
      Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
      Speaker: Victoria Quiett
      Presented at: 2023 Lab Compliance Connect
      13
      MAY 11, 2023 | 8:00 AM
      Benefits of Real-Time PCR Multiplexing
      Benefits of Real-Time PCR Multiplexing
      Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
      Speaker: John Pfeifer, PhD
      Sponsored By: Thermo Fisher Scientific
      22
      FEB 15, 2023 | 7:00 AM
      Scattering Microscopy: Label-Free Imaging Approaches
      Scattering Microscopy: Label-Free Imaging Approaches
      Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
      Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
      Sponsored By: Teledyne Photometrics
      28
    MAR 20, 2024 | 8:00 AM
    Introducing the first dual reporter application for Luminex xMAP INTELLIFLEX DR-SE Instruments:  measurement of phosphorylated and total proteins on the same bead
    Introducing the first dual reporter application for Luminex xMAP...
    Luminex and Thermo Fisher Scientific are excited to feature two presentations highlighting the advanced features of the Invitrogen™ Luminex® xMAP® INTELLIFLEX™ DR-SE inst...
    Speaker: Sherry Dunbar, Ph.D., MBA , Sigrun Badrnya, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    11
    DEC 12, 2023 | 10:00 AM
    Characterization of LNPs via Density Matching AUC
    Characterization of LNPs via Density Matching AUC
    Determining the purity of LNPs loaded with nucleic acids can be challenging due to their size heterogeneity. Here, we present analytical ultracentrifugation (AUC) methods that use multiwavel...
    Speaker: Amy Henrickson
    Sponsored By: Beckman Coulter Life Sciences
    11
     
     
    JUN 08, 2023 | 10:40 AM
    Analytical Validation for qPCR with Live Q&A
    Analytical Validation for qPCR with Live Q&A
    Real-time PCR, also known as quantitative PCR (qPCR), is the gold standard for sensitive, specific detection and quantification of nucleic acid targets. This qPCR technology as offered by Th...
    Speaker: Victoria Quiett
    Presented at: 2023 Lab Compliance Connect
    13
    MAY 11, 2023 | 8:00 AM
    Benefits of Real-Time PCR Multiplexing
    Benefits of Real-Time PCR Multiplexing
    Date: May 11, 2023 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics and vaccines....
    Speaker: John Pfeifer, PhD
    Sponsored By: Thermo Fisher Scientific
    22
    FEB 15, 2023 | 7:00 AM
    Scattering Microscopy: Label-Free Imaging Approaches
    Scattering Microscopy: Label-Free Imaging Approaches
    Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
    Speaker: Philipp Kukura , Francesco Reina , Matthew Kose-Dunn , Phil Allen
    Sponsored By: Teledyne Photometrics
    28
    SEP 22, 2022 | 10:00 AM
    Digital PCR basics
    Digital PCR basics
    Date: September 22, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Digital PCR (dPCR) is a specialized approach to nucleic acid detection ......
    Speaker: Dr. Dave Bauer, PhD
    Sponsored By: Thermo Fisher Scientific
    24 3
    SEP 07, 2022 | 12:00 PM
    A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting CRISPR-based Sample Depletion and Metagenomic Sequencing with Live Q&A
    A Universal "Day Zero" Infectious Disease Testing Strategy Exploiting...
    The lack of preparedness for detecting the highly infectious SARS-CoV-2 pathogen, the pathogen responsible for the COVID-19 disease, has caused enormous harm to public health and the economy...
    Speaker: Jon Armstrong, MSc
    Presented at: Microbiology Week Virtual Event Series 2022
    Sponsored By: Jumpcode Genomics
    5
    JUN 16, 2022 | 7:00 AM
    High-throughput LNP-mRNA integrity profiling
    High-throughput LNP-mRNA integrity profiling
    Date: June 16, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Lipid-based nanoparticles (LNPs) are effective non-viral vectors for delivering messenger RNA products (mRNA), most notab...
    Speaker: Jérémie Parot
    Sponsored By: SCIEX
    14
    MAY 25, 2022 | 10:00 AM
    A digital PCR-based protocol to detect and quantify RNA editing events at hotspots
    A digital PCR-based protocol to detect and quantify RNA editing...
    Date: May 25, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. P...
    Speaker: Rémi Buisson
    Sponsored By: Thermo Fisher Scientific
    22
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    Nucleic Acid Quantitation
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    Virtual Events

    • SEP 14, 2021  | 7:00 AM PDT
      Working in Tandem - 1st Annual TMT Symposium
      Working in Tandem - 1st Annual TMT Symposium
      Working in Tandem : 1st annual TMT Symposia Tandem Mass Tags (TMT) are isobaric labels that allow for simultaneous identification and quantification of protein expression from multiple sampl...
      17 1
      SEP 08, 2021  | 6:00 AM PDT
      xMAP® Connect Virtual Conference (On-Demand)
      xMAP® Connect Virtual Conference (On-Demand)
      Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
      17 1
      Webinar series: Simple steps to publication-quality western blots
      Webinar series: Simple steps to publication-quality western blots
      Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...
      24
      Accelerating diagnostics test development
      Accelerating diagnostics test development
      Each step in a diagnostic test's journey-from raw material extraction to patient administration-can influence outcomes....
      19
      JUN 08, 2021  | 7:00 AM PDT
      Shaping the Future of LC-MS in Life Science Together
      Shaping the Future of LC-MS in Life Science Together
      The global pandemic has not only changed our plans for how we live and work, but also accelerated the need for innovation to protect human health. Join us for an Innovation Summit to hear fi...
      26
    SEP 14, 2021  | 7:00 AM PDT
    Working in Tandem - 1st Annual TMT Symposium
    Working in Tandem - 1st Annual TMT Symposium
    Working in Tandem : 1st annual TMT Symposia Tandem Mass Tags (TMT) are isobaric labels that allow for simultaneous identification and quantification of protein expression from multiple sampl...
    17 1
     
     
    SEP 08, 2021  | 6:00 AM PDT
    xMAP® Connect Virtual Conference (On-Demand)
    xMAP® Connect Virtual Conference (On-Demand)
    Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
    17 1
    Webinar series: Simple steps to publication-quality western blots
    Webinar series: Simple steps to publication-quality western blots
    Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...
    24
    Accelerating diagnostics test development
    Accelerating diagnostics test development
    Each step in a diagnostic test's journey-from raw material extraction to patient administration-can influence outcomes....
    19
    JUN 08, 2021  | 7:00 AM PDT
    Shaping the Future of LC-MS in Life Science Together
    Shaping the Future of LC-MS in Life Science Together
    The global pandemic has not only changed our plans for how we live and work, but also accelerated the need for innovation to protect human health. Join us for an Innovation Summit to hear fi...
    26
    SEP 29, 2020  | 6:00 AM PDT
    Applied Biosystems Genetic Analysis Virtual Conference
    Applied Biosystems Genetic Analysis Virtual Conference
    Due to the fact that many large events and scientific conferences have been cancelled, we want to provide you with an outlet to present your research and learn from others in our community....
    28
    APR 10, 2019  | 6:00 AM PDT
    Molecular Diagnostics Virtual Event Series 2019
    Molecular Diagnostics Virtual Event Series 2019
    The 4th Annual Event in the Molecular Diagnostics Virtual Event Series is now available On Demand!...
    42 3
    FEB 27, 2019  | 6:00 AM PST
    Drug Discovery Virtual Event Series 2019
    Drug Discovery Virtual Event Series 2019
    Labroots and the Drug Discovery planning committee are pleased to announce its 2nd annual event in the Drug Discovery Virtual Event Series....
    40 1
    SEP 26, 2018  | 6:00 AM PDT
    Cell Biology Virtual Event Series 2018
    Cell Biology Virtual Event Series 2018
    Join us for the 2nd Annual Event in the Cell Biology Virtual Event Series, as we discuss recent discoveries in biological research, advancements in techniques...
    27 2
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