APR 04, 2016 6:21 AM PDT

Dual-Action Nanoparticles Report Cancer Cell Death in Real Time

WRITTEN BY: Xuan Pham
How do doctors and cancer patients know if their chemotherapy or radiation treatments are working to kill the tumor? Quite often, it is a tense process of wait-and-see. But a new technique could allow doctors to monitor cancer death in real time – an astonishing feat compared to the weeks and months of waiting for conventional treatments.
 
Nanoparticles target cancer cells and report back cell death

 Researchers at the Brigham and Women's Hospital (BWH) developed a method of tagging cancer cells as they die. This approach reportedly allows doctors to know the response of anticancer therapies within mere eight hours post treatments. And the technique holds promise for other chemotherapies and immunotherapies often used in cancer treatments. 

Their innovation rests on dual-action nanoparticles that deliver the drugs and report back the efficacy of the drugs in real time. The research team, led by Shiladitya Sengupta, leveraged the knowledge that specific caspase proteins are released when cells die. Thus, the nanoparticles has a ‘reporter element’ that glows green when they detect the presence of caspases after tumor cell deaths. The green fluorescent signals allow doctors to visualize treatment efficacy within hours after treatment and determine whether a tumor is resistant or susceptible to the drug.
 
"Using this approach, the cells light up the moment a cancer drug starts working. We can determine if a cancer therapy is effective within hours of treatment," said co-senior author Shiladitya Sengupta. "Our long-term goal is to find a way to monitor outcomes very early so that we don't give a chemotherapy drug to patients who are not responding to it."
 
To test the reporter system, the researchers filled the nanoparticles with paclitaxel, a common anticancer drug, in a pre-clinical model of prostate cancer. In parallel, they tested an immunotherapy agent against melanoma. Prostate tumors sensitive to paclitaxel had about 400 percent more fluorescent signals, as compared to paclitaxel-resistant tumors. Similarly, fluorescent signals were also significantly enhanced in drug-sensitive melanomas.
 
The experiments demonstrate that the dual-action nanoparticles can inform clinicians on the sensitivity state of tumors, thus allowing doctors to modify treatment plans accordingly sooner rather than later. According to the team, this is the first time that the anticancer actions of the nanoparticles have been captured in real time, in live organisms.
 
"We've demonstrated that this technique can help us directly visualize and measure the responsiveness of tumors to both types of drugs," said co-senior author Ashish Kulkarni. "Current techniques, which rely on measurements of the size or metabolic state of the tumor, are sometimes unable to detect the effectiveness of an immunotherapeutic agent as the volume of the tumor may actually increase as immune cells begin to flood in to attack the tumor. Reporter nanoparticles, however, can give us an accurate read out of whether or not cancer cells are dying."

Additional source: BWH press release
About the Author
Bachelor's (BA/BS/Other)
I am a human geneticist, passionate about telling stories to make science more engaging and approachable. Find more of my writing at the Hopkins BioMedical Odyssey blog and at TheGeneTwist.com.
You May Also Like
SEP 29, 2022
Cancer
Customer-Led Innovation: Building the CTS Xenon
Customer-Led Innovation: Building the CTS Xenon
How customers and R&D scientists came together across oceans and a pandemic to create a next-gen cell therapy soluti ...
OCT 11, 2022
Cell & Molecular Biology
Germ Cells Burrow Like Bulldozers
Germ Cells Burrow Like Bulldozers
In the early embryo, there is a cascade of cellular movement, and all of those cells have to move to the right position ...
NOV 19, 2022
Clinical & Molecular DX
New Research Shows How Bacteria Could Help Tumors Progress and Resist Treatment
New Research Shows How Bacteria Could Help Tumors Progress and Resist Treatment
New research from the Fred Hutchinson Cancer Center in Seattle postulates that certain bacteria significantly impact the ...
DEC 12, 2022
Cell & Molecular Biology
Preserve scRNAseq Sample Integrity and Biological Diversity: Enabling QC Cost Savings
Preserve scRNAseq Sample Integrity and Biological Diversity: Enabling QC Cost Savings
Separating single cells from their sample matrix without compromising cell integrity is a balancing act. Single cell RNA ...
JAN 16, 2023
Cancer
Conspiracy Theories and Perception of Cancer Risk
Conspiracy Theories and Perception of Cancer Risk
Experts describe conspiracy theories as explanations for important events involving secret plots by powerful, malicious ...
JAN 25, 2023
Cancer
Pregnancy After, or During, Breast Cancer
Pregnancy After, or During, Breast Cancer
Breast cancer remains the most common cancer in women in the United States.  Experts estimated 287,850 breast cance ...
Loading Comments...