NOV 08, 2018 11:59 AM PST

Ancient Animal Provides a New Window Into Tissue Cohesion

WRITTEN BY: Carmen Leitch

Stanford University bioengineer Manu Prakash wanted to visualize the behavior of every cell in an adult organism, simultaneously and in detail. To do so, he found a marine animal that is considered one of the simplest, Trichoplax adhaerens, which he gave the nickname Tplax. He began to study it almost eight years ago, and since then it has revealed a new kind of contraction between its cells that may help us understand how complex animals withstand the forces generated by the body and the environment. These findings, which were reported in the Proceedings of the National Academy of Sciences, may inspire the creation of a special material called an active solid that can rapidly make significant alterations to its own physical nature.

“Much of the rules of biology that we read in textbooks have been, so far, dictated by a few sets of model organisms,” said Prakash, who is an associate professor of bioengineering and senior study author. “If we intend to be the generation that will unravel laws of biology, it’s extremely important to understand and appreciate the diversity of what has evolved on our planet and think much more holistically about what is actually possible in biological systems.”

The researchers had to learn how to work with Tplax, which moved around a lot under their microscopes. Eventually, they were able to track every adjustment in its cells.

“There was literally a day where, for the first time, I had some of the stains that label Tplax cells working, and under the microscope, we saw an explosion of cellular contractions,” recalled Prakash. “It looked like fireworks under a microscope, and that was the moment that told us there is something very special about this animal and we needed to understand it.”

The fast contractions of Tplax cells were generating those fireworks. Its body is composed of two layers of tissue that are like skin. It has no neurons or muscles, but the researchers found that in its epithelial cell layer, contractions are happening that are ten times faster than any ever reported in epithelial cells. The force from those squeezes would rip apart biological tissue that was comparably thin - only a quarter of the thickness of a piece of paper. 

The researchers have hypothesized that a mechanism called active cohesion prevents the tissue from tearing - while some cells are strongly contracting, others are softening. Instead of reacting to a force, there is coordination to the cell movement, distributing stress throughout Tplax and allowing the animal to remain in control, and intact.

These findings (which are outlined in the video above) open up new questions about whether cellular activity works in concert with epithelial contractions.

“We look at this simple creature and we see it make decisions and move and hunt,” said lead study author Shahaf Armon, a Prakash lab postdoctoral fellow. “It’s a huge evolutionary question, how single cells merged to become multicellular organisms and how such a minimal tissue made of identical cells is able to then perform complex behaviors.” 

The team wants to use this knowledge to engineer an active solid. They know that the Tplax epithelium has a unique structure; cells shaped like T’s have a thin top sheet and a nucleus at the bottom, which are lined up like a layer of bricks. That geometry could be a critical part of a new material. 

By utilizing laboratory lineages, the researchers were also able to generate Tplax with very different sizes and shapes. Cell coordination can thus be modeled on many levels with those animals.

“Tplax are really mysterious beasts,” added study co-author Matthew Bull, a graduate student in the Prakash lab, “but we use that to our advantage to find where our understanding of what it means to be part of the animal kingdom bends and then breaks.”

The lab discusses these findings in the video.


Source: Stanford University, PNAS

About the Author
  • Experienced research scientist and technical expert with authorships on over 30 peer-reviewed publications, traveler to over 70 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
SEP 11, 2020
Immunology
Study Reveals Tumor Defense Mechanism... And How to Beat It
SEP 11, 2020
Study Reveals Tumor Defense Mechanism... And How to Beat It
  P53 is an infamous process gene at the core of the development of tumors.  When P53  functional, it pau ...
OCT 01, 2020
Cancer
Understanding in vivo Metabolomics: C13 Isotope Studies
OCT 01, 2020
Understanding in vivo Metabolomics: C13 Isotope Studies
One key to understanding cancer metabolomics lies in the ability to accurately replicate the natural environment of the ...
OCT 26, 2020
Cancer
Investigating the Receptor Protein FPR1 in Brain Cancer
OCT 26, 2020
Investigating the Receptor Protein FPR1 in Brain Cancer
Amongst the more common targets for cancer therapies are cell surface receptors. These receptors are proteins – us ...
OCT 29, 2020
Genetics & Genomics
Severe Genomic Damage in Human Embryos Treated With CRISPR
OCT 29, 2020
Severe Genomic Damage in Human Embryos Treated With CRISPR
The CRISPR-Cas9 genomic editing system holds great promise for treating genetic errors that cause human disease. But we ...
NOV 19, 2020
Cardiology
Examining a Possible Link Between Atherosclerosis and Inflammation
NOV 19, 2020
Examining a Possible Link Between Atherosclerosis and Inflammation
The immune system’s primary duty is to protect the body from dangerous invaders. Many don’t know that it als ...
NOV 17, 2020
Cell & Molecular Biology
Chronic Inflammation Lowers Levels of Aging-Linked Molecule
NOV 17, 2020
Chronic Inflammation Lowers Levels of Aging-Linked Molecule
Over the years, the molecule nicotinamide adenine dinucleotide (NAD+) has gone from being a player in some biochemical p ...
Loading Comments...