MAR 24, 2019 01:38 PM PDT

Getting a Better Look at Patterns in Layers of Cells in the Eye

WRITTEN BY: Carmen Leitch

The eye contains complex layers of cells, one of which is called retinal pigment epithelium (RPE). Scientists at the National Eye Institute (NEI, a part of the National Institutes of Health) have combined a fluorescent dye with adaptive optics to observe individual cells in that critical layer. This technique can aid in the treatment of diseases that impact the RPE, like retinal degeneration. It was used in this work to compare patterns in the RPE cells of healthy individuals with patterns in retinal disease patients. The findings have been reported in JCI Insight and is summarized in the video.

"Studying cells of the retinal pigment epithelium in the clinic is like looking into a black box. RPE cells are difficult to see, and by the time signs of disease are detectable with conventional techniques, a lot of damage has often already occurred," explained the lead author of the work Johnny Tam, Ph.D. "This study is proof-of-concept that we can use a fluorescent dye to reveal this unique fingerprint of the RPE, and to monitor the tissue over time."

Cells in the RPE are adjacent to photoreceptors, which carry pigment and can absorb light that hits them. That means that imaging the thin RPE cell layer is challenging, even with adaptive optics. Tam decided to apply an FDA-approved dye called indocyanine green (ICG). The fluorescent dye is used to visualize blood vessels in the back of the eye and leaves those blood vessels in about thirty minutes. ICG remains in the RPE for a few hours though, and reveals a detectable pattern; some cells are dim while others are bright.

"Initially, we didn't know how the dye was going to look," said Tam. "We put the dye in, and we got this pattern that at first looked kind of random. It was a big surprise that we could come back after a year, re-inject the dye, and see the same pattern."

Tam’s team created software to assess patterns in the RPE cells and changes in those patterns, which didn’t change much in healthy people over several months.

Late-onset retinal degeneration (L-ORD) is thought to impact RPE cells in later disease stages. In an L-ORD patient in the early stages of the disease, the RPE was only a bit less stable than those in healthy people. 

Mosaic of retinal pigment epithelial cells, visualized with indocyanine green (ICG) and adaptive optics. / Credit: Johnny Tam, Ph.D., NEI

Bietti crystalline dystrophy (BCD) is a disease that causes the progressive loss of RPE cells. The researchers found that in a patient with BCD, RPE cells were larger and disorganized compared to healthy cells overall. There were major changes in the pattern of RPE cells impacted by BCD over time.

Tam is hopeful that the RPE patterns can be observed with conventional imaging methods eventually. That can help scientists understand dysfunction in the layer and develop ways to repair it.

"When treating patients, a lot of decisions are based on what we see. For the parts of the eye we can't see, we're often treating blindly; this technique will show us what's happening in the tissue over time, helping us develop new treatments for these conditions," concluded Tam.

The layers of the retina include the RPE. / Illustration: Lisa Birmingham


Sources: AAAS/Eurekalert! Via NEI, JCI Insight

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
NOV 15, 2019
Cell & Molecular Biology
NOV 15, 2019
How the Kava Plant Creates Medicinal Compounds
Nature has given us some of our best medicines; it's thought that as many as half the drugs we used are derived from natural products....
NOV 15, 2019
Cell & Molecular Biology
NOV 15, 2019
How Proteins Send Instant Messages
Our cells use proteins as messengers that send or receive critical signals to carry out the functions essential for life....
NOV 15, 2019
Cell & Molecular Biology
NOV 15, 2019
RNA Polymerases Can Signal to One Another Over Long Genomic Distances
Scientists have taken a close look at transcription in the Escherichia coli bacterium at the level of a single molecule....
NOV 15, 2019
Drug Discovery & Development
NOV 15, 2019
Tailor-made Drug for Girl with Rare Genetic Disease
Researchers have used personalized medicine to develop a tailor-made genetic treatment for a girl diagnosed with a rare genetic disease known as Batten dis...
NOV 15, 2019
Cell & Molecular Biology
NOV 15, 2019
Discovery of Cell's Mechanism for Sensing Oxygen Wins Nobel
It may be possible to attack cancer or treat anemia or other diseases by interfering with the cells' ability to sense oxygen....
NOV 15, 2019
Cell & Molecular Biology
NOV 15, 2019
The Different Signals Sent by Brown and White Fat
Scientists have now learned more about the molecules that brown fat releases....
Loading Comments...