JUL 10, 2016 05:40 AM PDT

Insect Analogue for Intermediate Filaments Discovered

WRITTEN BY: Carmen Leitch
Intermediate filaments (IFs) are critical parts of the structure of cells. They are essential for both normal tissue structure and function. Enabling cells to withstand the stretch and pressure on the tissues in which they reside, they protect against problems like blistering and cataracts. Found in the nucleus (lamins) and the cytoplasm (cytoplasmic intermediate filaments), they take their name from their filament diameter.
High magnification view of the filaments (in red) made by the newly discovered atypical tropomyosin shows that they do not overlap with actin filaments (in green). / Credit: UCSB/Cell Cho et al
Strangely, they have not been found in insects, and it was thought that an unknown group of proteins had taken on this function in those organisms. 

Publishing in Cell Reports, new work conducted by biologist Denise Montell and her team of researchers at the University of California Santa Barbara (UCSB) may have the answer. They have discovered a protein that has IF-like characteristics in fruit flies. They observed that this unusual form of a protein called tropomyosin was in every cell type they analyzed. 

"Believe it or not, fruit flies and humans have a lot of proteins in common, including conventional tropomyosin," explains Montell, the Robert and Patricia Duggan Chair of Mathematical, Life, and Physical Sciences in UCSB's Department of Molecular, Cellular, and Developmental Biology.

"Evolution started with tropomyosin and morphed it into a brand new protein with very different characteristics simply by adding a few bits to each end," she continues. "Like IFs -- but unlike normal tropomyosin -- this new protein makes filaments that are 'intermediate' in size. This means they are larger than F-actin filaments and smaller than microtubules, two other important structural filaments in cells." This newly discovered type of filament-forming protein may be analogous to some intermediate filaments in flies.
A graphical comparison of insect tropomyosins and neurofilaments
Normal tropomyosin creates a rope-like structure, called coiled coil. This thin tropomyosin coil normally winds around F-actin filaments. However, this newly discovered version doesn't resemble or behave like a normal tropomyosin and is coiled in only one small region. "We knew what it wasn't, but we wanted to know what it was," says Montell. 

The team utilized a computer program which examined the uncoiled, disordered regions at each end of the protein to better understand the function of this unique molecule. 

"While this configuration -- coiled coil with intrinsically disordered domains at each end -- resembled IF proteins generally, the novel tropomyosin differed from IFs in the details," Montell explains. "Still, we wondered whether this atypical protein might have filament-making capabilities or the ability to perform IF-like functions."

A colleague at the University of Texas Southwestern Medical Center, Masato Kato, made the protein in a tube to investigate conditions such as salt concentration and pH that are appropriate for different types of IF proteins. He determined that the environment in which this new protein from flies formed 13- to 16-nanometer-wide filaments had never previously been described.

"We found all kinds of similarities in biochemical behavior between this protein and IF proteins despite the differences in sequence," Montell says. "Because our protein seems to have some properties in common with IFs, it may be the answer to the long-standing question of how flies survive without actual IF proteins. But like any exciting discovery, it also raises many more questions."

Sources: AAAS/Eurekalert! via UCSB, Cell Reports
About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
SEP 18, 2019
Cell & Molecular Biology
SEP 18, 2019
Could we be allergic to CRISPR?
A new study published in Nature reveals an immunity to the CRISPR-Cas9 proteins in humans...
SEP 18, 2019
Cell & Molecular Biology
SEP 18, 2019
Learning More About the Causes of Endometriosis Pain
Endometriosis can be an extremely painful disorder that significantly impacts quality of life....
SEP 18, 2019
Genetics & Genomics
SEP 18, 2019
In a First, Sickle Cell Patient Receives CRISPR Gene Therapy
Many human diseases can be traced back to genetic mutations, and researchers are beginning to find ways to correct those errors....
SEP 18, 2019
Immunology
SEP 18, 2019
A Food Additive Could Make Your Cat Hypoallergenic
With 10-30% of the population reporting sensitivity to cat dander, you probably know someone with a cat allergy.  That's why scientists at Nestl&e...
SEP 18, 2019
Cell & Molecular Biology
SEP 18, 2019
A High-fat Diet Can Trigger Changes in the Brain
We all need some fat in our diets, but eating excess amounts of unhealthy fat has been linked to poor health outcomes....
SEP 18, 2019
Genetics & Genomics
SEP 18, 2019
Rearranging Whole Chromosomes with CRISPR
CRISPR/Cas9 was developed as a gene-editing tool and now it's going beyond small, targeted changes in the genome....
Loading Comments...