AUG 06, 2016 07:06 AM PDT

Method for Measuring Methylation Levels of RNA Developed

WRITTEN BY: Carmen Leitch
4 4 625
A new sequencing technique that seeks to reveal important details about RNA has been developed by investigators at UCLA. Published in Nature Methods, it can measure for each gene, what percentage of RNA has a chemical modification called methylation. This could be a very beneficial tool for stem cell research, as methylation levels influence the differentiation or specification of stem cells into more specialized cells.
m6A modification of transcripts allows stem cells to effectively
RNA is the intermediary between the genome and the resulting proteins that make up an organism. While the genome contains the information, the RNA orchestrates the activities of the cell to carry out the processes. After DNA is transcribed into RNA, it undergoes processing that has an impact on the resulting proteins.

How methylation influences RNA activity is not well understood. There are several types of methylation, this study focuses on the kind that is known as m6A or N6-methyladenosine.  A range of functions results from this processing, for example, the length of time the RNA remains in the cell before degradation, or the amount of protein it will make. The m6A modification is, importantly, the most abundant methylation type on RNAs that produce protein. 
GO enrichment analyses of top 1,000 genes with highest methylation levels (purple) and bottom 1,000 genes with least methylation (orange) / Credit: Nature Methods Molinie et al
The sequencing method newly described by the authors is called m6A-LAIC-seq. "Previously, we were only able to determine the location of m6A on the RNA, but not the amount," explained Yi Xing, a Professor of Microbiology, Immunology and Molecular Genetics and a member of the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA.
Pluripotent stem cells hold such great promise for therapeutics because they are able to turn into any specific type of cell in the body, such as liver, blood, brain or skin cells through a process called differentiation. In addition, they’re able to make copies of themselves. Check out the video above to know more about the basics of stem cells.

Knowing what percentage of RNA has the m6A modification provides information that may help detect disease, said Xing, because diseased and healthy cells could have varying m6A levels. Researchers can also use information about RNA m6A levels to help answer questions about stem cell differentiation, something of particular interest to scientists investigating regenerative medicine. Another possibility for the application of this work is helping researchers maintain stem cells in culture. They have a tendency to spontaneously differentiate, hindering research due to the loss of control of cell fate. A team led by Xing and Cosmas Giallourakis has shown previously that blocking m6A stops pluripotent stem cells from differentiating, keeping them in a suspended state while remaining healthy.

"We are very excited about the promising data and the new tool that is now available to study m6A in a wide range of cell types including pluripotent stem cells," Xing said. "We anticipate that our research will improve the understanding and use of pluripotent stem cells in regenerative medicine."
In the video above, Xing gives a talk on his work at the Institute for Pure and Applied Mathematics, UCLA.

Sources: Phys.org via UCLA, Nature Methods, Cell Stem Cell
About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
JUN 12, 2018
Cell & Molecular Biology
JUN 12, 2018
Loss of Brain Lesions may Indicate Worsening MS
New research could help doctors determine how severe a case of multiple sclerosis will become.
JUN 13, 2018
Cell & Molecular Biology
JUN 13, 2018
Concerns About the CRISPR/Cas9 Gene Editor
Hailed as a revolutionary technique that had many potential applications, two new studies show that we should proceed with caution.
JUN 16, 2018
Cell & Molecular Biology
JUN 16, 2018
Watching the Movement of Stem Cells
Video is changing the way data is collected in the lab.
JUN 27, 2018
Immunology
JUN 27, 2018
Immune Cells Responsible for Chemo-induced Diarrhea
While studying specific immune cells in the context of chronic itching in the skin, two Washington University School of Medicine scientists discovered that
JUN 30, 2018
Genetics & Genomics
JUN 30, 2018
Gene-editor Eases Autism Symptoms in Mouse Model
This is the first time a gene alteration has successfully changed behaviors associated with autism.
JUL 07, 2018
Genetics & Genomics
JUL 07, 2018
Cause of Gender Differences in Neurodevelopmental Disorders is ID'ed
Gender is a known factor in the development of neurological disorders. Now scientists think they know why.
Loading Comments...