FEB 14, 2016 6:22 AM PST

Bacterial Infection Detection in Under One Minute

WRITTEN BY: Xuan Pham
Pseudomonas aeruginosa, a bacteria that commonly infects chronic wounds

In the clinical setting, wound infections can significantly delay recovery time and even become life threatening. Early detection of the infection is crucial, but current methods can take over 24 hours to deliver a diagnosis. However, a new system of infection detection dramatically eliminates the wait time to just under a minute.
 
Traditional methods of detection of wound infections involve old-fashioned techniques of bacterial culture and overnight incubations. Even the more advanced methods that rely on molecular and biochemical identification still require several hours of incubation to obtain enough cells for analysis.
 
The newest method, developed by researchers at Northeastern University College of Engineering and the J. Craig Venter Institute, takes bacterial detection to the 21st century. Instead of bacterial cultures, the method uses a disposable electrochemical system that detects biomolecules released by the bacteria. More specifically, the system detects pyocyanin, a unique metabolite released by Pseudomonas aeruginosa, a bacteria that commonly infects chronic wounds. Doctors would only have to sample a small amount of wound fluid for infection diagnosis that takes less than 1 minute.
 
"Being able to detect Pseudomonas and other infectious organisms at the time of the clinic visit will greatly enhance our ability to take care of patients," said Victoria Shanmugam, co-author of the study. "We would not have to wait for culture results before making a decision about antibiotics, and this would allow us to better tailor therapies for our patients."
 
In comparing the results from the electrochemical assay to rRNA profiling via pyrosequencing, the researchers reported a sensitivity of 71% and specificity of 57% for detection of Pseudomonas bacteria.
 
Admittedly the results have room for improvements. But, perhaps the research could  be taken as a preliminary proof-of-concept study. Viewed as such, the study demonstrates success using a probe that rapidly detected unique bacterial molecules to detect infection. The disposable detection device provides a means for rapid point-of-care diagnosis directly at the patient’s bedside. With improvements and extension to include probes for other bacteria, doctors can be informed to treat infected patients with specific antibiotics sooner, reducing long-term health risks and improving overall patient outcome.
 
"Infections are a major challenge in medicine, and by using this probe, we were able to harness one of the unique molecules produced by bacteria to detect infection," said Shanmugam. "Through this ongoing collaboration with Dr. Goluch's team of engineers, we plan to continue to refine this testing method and hope to scale it up for detection of other bacteria and to optimize it for clinical use."
 
Additional source: EurekAlert!
 
About the Author
  • I am a human geneticist, passionate about telling stories to make science more engaging and approachable. Find more of my writing at the Hopkins BioMedical Odyssey blog and at TheGeneTwist.com.
You May Also Like
APR 15, 2021
Clinical & Molecular DX
A Portable Ebola Detector, 1000x More Sensitive Than Lab-Based Tests
APR 15, 2021
A Portable Ebola Detector, 1000x More Sensitive Than Lab-Based Tests
Duke University scientists have created a highly sensitive Ebola virus portable diagnostic device, 1000 times more sensi ...
MAY 03, 2021
Health & Medicine
The Challenges and Advantages of Direct PCR Amplification
MAY 03, 2021
The Challenges and Advantages of Direct PCR Amplification
  Testing for COVID-19 continues to be one of the most important tools in the global fight to slow and reduce the i ...
MAY 14, 2021
Microbiology
An Imaging Tool for Seeing Bacterial Infections in the Body
MAY 14, 2021
An Imaging Tool for Seeing Bacterial Infections in the Body
Researchers have created an imaging agent that will allow scientists and clinicians to visualize a bacterial infection c ...
JUL 19, 2021
Cardiology
Immune Proteins & Blood Clots May be Connected to Psychosis Development
JUL 19, 2021
Immune Proteins & Blood Clots May be Connected to Psychosis Development
Researchers are pointing out the associations between blood clotting disruptions and the front line immune system, and t ...
AUG 04, 2021
Clinical & Molecular DX
Fear of Smells Could Help Diagnose Migraines in Kids
AUG 04, 2021
Fear of Smells Could Help Diagnose Migraines in Kids
  A new study suggests the intense dislike of pungent smells and odors could be a diagnostic marker for migraines a ...
AUG 17, 2021
Clinical & Molecular DX
Delays in Breast Cancer Diagnoses Among Black Women
AUG 17, 2021
Delays in Breast Cancer Diagnoses Among Black Women
Thanks to breakthrough diagnostic technologies, we can now catch the early warning signs of breast cancer much faster th ...
Loading Comments...