FEB 15, 2016 03:53 PM PST

Single-Cell RNA Sequencing Facilitates New Immune Cell Discovery

WRITTEN BY: Kara Marker
The mucosal lining of places in the body like the lungs and intestines is the first line of defense against pathogens the body encounters. Certain components of the mucosa known vaguely as innate lymphoid cells (ILCs) help to maintain the mucosal lining, but little else is known for sure about their immune function. Nature refers to ILCs as “immune cells that belong to the lymphoid lineage but do not express antigen-specific receptors.” In a new study from the Karolinska Institutet in Sweden, scientists were able to characterize a certain subgroup of cells called ILC3s into three further subtypes, and they believe this discovery is the next piece of the mystery puzzle surrounding ILCs.
 


Researchers from this study, published recently in Nature Immunology, used single-cell RNA sequencing to examine the unique gene expression profiles of human tonsil cells, also called the transcriptome. Single-cell RNA sequencing is becoming increasingly popular as a technique for “profiling rare or heterogeneous populations of cells” (Nature Methods).
 
As expected, the information gathered from the individual tonsil cells showed three main groups of ILCs (ILC1, ILC2, and ILC3) as well as the gene expression levels of natural killer cells. However, their analysis also found three previously unknown subgroups of ILC3. These three subgroups differed based on protein secretion and molecular sequencing, proving to be “transcriptionally and functionally diverse subpopulations of ILC3 cells” (Nature Immunology).
 
“Our analyses also discovered the expression of numerous genes of previously unknown function in ILCs, highlighting that these cells are likely doing more than what we previously knew,” said Rickard Sandberg, PhD.
 
 
Source: Karolinska Institute
 
About the Author
  • I am a scientific journalist and enthusiast, especially in the realm of biomedicine. I am passionate about conveying the truth in scientific phenomena and subsequently improving health and public awareness. Sometimes scientific research needs a translator to effectively communicate the scientific jargon present in significant findings. I plan to be that translating communicator, and I hope to decrease the spread of misrepresented scientific phenomena! Check out my science blog: ScienceKara.com.
You May Also Like
SEP 26, 2018
Videos
SEP 26, 2018
Cancer Immunotherapy
Video illustration about how tumor cells are sensed and destroyed by the immune system and how tumors evolve and detect immune-mediated eliminations, as well as iimmunotherapies associated....
OCT 01, 2018
Immunology
OCT 01, 2018
Fate of the T Cell
The immune system consists of a variety of different cell types. But how do the cells undergo differentiation? A study recently published in Science provides insight....
OCT 11, 2018
Cell & Molecular Biology
OCT 11, 2018
Revealing a 'Double Agent' in the Immune System
Researchers want to enhance our natural defenses to fight a variety of health problems more effectively....
NOV 21, 2018
Immunology
NOV 21, 2018
HIV Persisting How?
A team of researchers have identified an HIV reservoir...
DEC 18, 2018
Health & Medicine
DEC 18, 2018
Mapping the Human Proteome
Advancing our understanding of human disease often requires a sound understanding of normal human physiology. A critical tool to provide guidance on human...
DEC 21, 2018
Microbiology
DEC 21, 2018
How the Gut Microbiome Controls the Intestinal Immune System
The gut microbiome has many important functions, including helping us digest food. But it has to protect itself from the immune system....
Loading Comments...