FEB 15, 2016 03:53 PM PST

Single-Cell RNA Sequencing Facilitates New Immune Cell Discovery

WRITTEN BY: Kara Marker
The mucosal lining of places in the body like the lungs and intestines is the first line of defense against pathogens the body encounters. Certain components of the mucosa known vaguely as innate lymphoid cells (ILCs) help to maintain the mucosal lining, but little else is known for sure about their immune function. Nature refers to ILCs as “immune cells that belong to the lymphoid lineage but do not express antigen-specific receptors.” In a new study from the Karolinska Institutet in Sweden, scientists were able to characterize a certain subgroup of cells called ILC3s into three further subtypes, and they believe this discovery is the next piece of the mystery puzzle surrounding ILCs.
 


Researchers from this study, published recently in Nature Immunology, used single-cell RNA sequencing to examine the unique gene expression profiles of human tonsil cells, also called the transcriptome. Single-cell RNA sequencing is becoming increasingly popular as a technique for “profiling rare or heterogeneous populations of cells” (Nature Methods).
 
As expected, the information gathered from the individual tonsil cells showed three main groups of ILCs (ILC1, ILC2, and ILC3) as well as the gene expression levels of natural killer cells. However, their analysis also found three previously unknown subgroups of ILC3. These three subgroups differed based on protein secretion and molecular sequencing, proving to be “transcriptionally and functionally diverse subpopulations of ILC3 cells” (Nature Immunology).
 
“Our analyses also discovered the expression of numerous genes of previously unknown function in ILCs, highlighting that these cells are likely doing more than what we previously knew,” said Rickard Sandberg, PhD.
 
 
Source: Karolinska Institute
 
About the Author
  • I am a scientific journalist and enthusiast, especially in the realm of biomedicine. I am passionate about conveying the truth in scientific phenomena and subsequently improving health and public awareness. Sometimes scientific research needs a translator to effectively communicate the scientific jargon present in significant findings. I plan to be that translating communicator, and I hope to decrease the spread of misrepresented scientific phenomena! Check out my science blog: ScienceKara.com.
You May Also Like
NOV 14, 2019
Cannabis Sciences
NOV 14, 2019
Can you be Allergic to Cannabis?
Pollen allergies are very common. As cannabis flowers produce, it is also possible to be allergic to cannabis. Here's what to look out for....
NOV 14, 2019
Health & Medicine
NOV 14, 2019
Why does the flu virus thrive during winter?
If you’re one of the nearly 7 million people who suffered from influenza last season, you might be curious why the virus hits so much during winter m...
NOV 14, 2019
Drug Discovery & Development
NOV 14, 2019
Novel Treatments for Auto-immune Disorders
A recent research study examined a library of almost 300,000 small molecules to search for a molecule that may be a potential target for the human GMP-AMP ...
NOV 14, 2019
Immunology
NOV 14, 2019
Your Immune Response Varies from AM to PM
“My biological clock is ticking.” We’ve heard people say this phrase - maybe even said it ourselves - but what do we mean exactly? Often...
NOV 14, 2019
Immunology
NOV 14, 2019
Treating Alzheimer's? Target Microglia
The body’s own immune cells may be the common denominator in Alzheimer’s disease. In a new study of the relationship between microglia, tau pro...
NOV 14, 2019
Microbiology
NOV 14, 2019
Antibody Discovered That May be the Key to a Universal Flu Vaccine
Instead of designing a new flu vaccine every year, researchers have made a breakthrough that may lead to a single vaccine that protects against all strains....
Loading Comments...