OCT 09, 2016 7:33 AM PDT

The Why & How of Microbial Cell Phenomics

WRITTEN BY: Carmen Leitch

In this talk presented by the American Society for Microbiology with Barry Bochner of Biolog, Inc, the hows and whys of analyzing the properties of an organism in its environment or phenotyping, are discussed.

So why should we phenotype cells of microbes? Observing the characteristics of microbes can help researchers understand how those properties might be affected by environmental pressures, genetics and epigenetics. Phenomics is a method for the study of phenotypic characteristics on a high-throughput scale, and is a way for scientists to avoid having to assay microbial colonies one by one, a tedious and inefficient process.

Bochner describes a simple way to assay the myriad complex biochemical pathways that are at work inside of a microbial cell. What seems like an impossible task has been reduced to a colorimetric test that is described in the video. The energy output of cells, growing in wells, is ascertained, with some wells containing inhibitors that can serve as a readout for many different pathways and functions. This phenotyping technology has been used in hundreds of publications over the past decade.

Bochner hopes to see more applications of this technique, such as in the analysis of human cells and in microbiome studies.
About the Author
BS
Experienced research scientist and technical expert with authorships on over 30 peer-reviewed publications, traveler to over 70 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
NOV 06, 2020
Space & Astronomy
300 Million Planets in the Milky Way May Be Habitable
NOV 06, 2020
300 Million Planets in the Milky Way May Be Habitable
Researchers have found that there could be at least 300 million habitable worlds in the Milky Way galaxy. This could mea ...
DEC 03, 2020
Chemistry & Physics
3-D Printed, Realistic Heart Model for Training Future Physicians
DEC 03, 2020
3-D Printed, Realistic Heart Model for Training Future Physicians
Building a realistic tissue model is critical for training young physicians and surgeons, and yet challenging due to the ...
DEC 29, 2020
Space & Astronomy
Why is the Sun's Outer Atmosphere Hotter than its Surface?
DEC 29, 2020
Why is the Sun's Outer Atmosphere Hotter than its Surface?
The fact that the Sun's distant atmosphere is significantly hotter than its surface has been a topic of confusion in ...
JAN 08, 2021
Neuroscience
Brain Scans Show Why Comedy-News Shows are More Memorable
JAN 08, 2021
Brain Scans Show Why Comedy-News Shows are More Memorable
Comedy-news shows such as those by Trevor Noah, John Oliver, and Samantha Bee on the rise and are especially popular amo ...
MAR 26, 2021
Plants & Animals
Studies Confirm that Bottlenose Dolphins Vocalize to Synchronize Behaviors
MAR 26, 2021
Studies Confirm that Bottlenose Dolphins Vocalize to Synchronize Behaviors
The ability to communicate with one another to coordinate behaviors contributes to the success of social mammals, like b ...
MAY 15, 2021
Space & Astronomy
Earth's Core has 70 Times More Hydrogen than the Oceans
MAY 15, 2021
Earth's Core has 70 Times More Hydrogen than the Oceans
Researchers from the University of Tokyo have found that under certain extreme conditions, hydrogen particles can bond s ...
Loading Comments...