MAR 23, 2017 03:30 PM PDT

PCR Primer Design for Cloning by Restriction Enzyme

WRITTEN BY: Carmen Leitch

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to amplify fragments of genetic material. In some cases, that material might then be inserted or cloned into a vector, another common molecular tool. Vectors come in many variations and can be used for a variety of purposes, and it's easy to manipulate the genetic sequence of interest once it's been cloned into a vector.

Restriction enzymes, yet another tool in the molecular kit, make cuts at specific, short genetic sequences. Restriction enzyme sequences can be added onto the ends of a genetic sequence through PCR, and they have been conveniently packed into vectors so genes can be easily inserted by using them. When using them to clone some DNA into a vector, the restriction enzymes have to be chosen carefully. Of course, it's important that they not cut the sequence of interest. They also should work well together. Catch a few more pointers from the short video.
About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
NOV 06, 2018
Plants & Animals
NOV 06, 2018
Why Bald Eagles Have Such Great Vision
The bald eagle is an incredible creature, not just for its beauty, but also because of its astonishing eyesight capabilities. Experts estimate that the bal...
NOV 14, 2018
Videos
NOV 14, 2018
Visualizing Embryonic Development in Zebrafish
Zebrafish share genetic features with many other organisms, and they undergo a rapid developmental process in which they can remain transparent....
NOV 20, 2018
Videos
NOV 20, 2018
A Major Grant Aims to Improve our Understanding of Age-related Cognitive Decline
The American Heart Association has teamed up with the Allen Initiative in Brain Health and Cognitive Impairment to award the Salk Institute $19.2 million....
NOV 26, 2018
Health & Medicine
NOV 26, 2018
Nontuberculous Mycobacterial Infections in Tattoos
Contracting an infection when getting a tattoo is always a major concern. Consumers should be aware of the risk of developing infections with bloodborne pa...
NOV 27, 2018
Videos
NOV 27, 2018
The Challenge of Creating a Vaccine for HIV
The CDC estimates that 1.1 million people in the US have HIV, and around 15% are unaware they have it....
DEC 05, 2018
Videos
DEC 05, 2018
Male Contraceptive Gel is in Clinical Trials
Researchers have started a clinical trial involving 420 participants to test the efficacy of a male contraceptive gel....
Loading Comments...