AUG 14, 2016 09:01 PM PDT

PCR Primer Design Pointers

WRITTEN BY: Carmen Leitch

In this video from Thermo Fisher Scientific, a few tips for better PCR primer design are presented. PCR, Polymerase Chain Reaction, is a common and vital part of genetic studies, biomedical research and medicine in general.

Considerations important to the creation of good primers, such as primer length and melting temperature of the primers are highlighted in the video.

In PCR, the reaction mixture cycles through a series of temperatures. One of those, the annealing temperature, is also related to specificity and can be varied if needed for troubleshooting. For example, a PCR reaction that produces non-specific amplification products can likely tolerate a higher, more stringent annealing temperature. If no product is observed following the PCR reaction, the annealing temperature could be lowered as one way of trying to generate a successful reaction product.

Some pitfalls of PCR primers are shown too, such as hairpins that can form in primers that have sequences amenable to bending such that ends of the primer anneal to each other. Another thing to avoid are long sequences of the same base, or primers that have ends with a lot of cytosine (C) or guanine (G) bases.
About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
NOV 18, 2019
Chemistry & Physics
NOV 18, 2019
Catastrophic Ancient Events Might Have Forever Changed Life-friendly Venus
There is plenty of evidence suggesting that Venus had a long history of being a habitable planet due to its abundance of water, plate tectonics, and amicab...
NOV 18, 2019
Plants & Animals
NOV 18, 2019
Why Do Camels Have Humps?
Camels are predominantly known for the humps that appear on their backs, and believe it or not, those humps are filled with body fat. Some camels sport jus...
NOV 18, 2019
Earth & The Environment
NOV 18, 2019
The Amazon is Still Burning
Although other crises may be dominating international news headlines, the Amazon rainforest is still burning. The Wall Street Journal stated that more than...
NOV 18, 2019
Earth & The Environment
NOV 18, 2019
Yale Scientists Solve Mass Extinction Mystery
Scientists from Yale University have solved a mystery that has plagued scientists for decades. It’s well established that 66 million years ago, a mas...
NOV 18, 2019
Space & Astronomy
NOV 18, 2019
What You Need to Know About NASA's Upcoming WFIRST Space Telescope
There are several space telescopes observing the cosmos at the very time of this writing, and there are plans to launch even more of them in the near futur...
NOV 18, 2019
Plants & Animals
NOV 18, 2019
Polar Bears Feast On Anything They Find, Even Rotting Whales
Polar bears are expert survivalists. Their noses are many times more powerful than a dog’s, and they can sniff out a potential meal from several mile...
Loading Comments...