OCT 15, 2019 10:00 AM PDT

The 'Three Peak Challenge' for long-read, ultra-deep stool metagenomics on the PromethION

Sponsored by: Zymo Research
Speakers

Abstract

DATE: October 15, 2019

TIME: 10:00am PDT

At present, most metagenomic surveys rely on high-output platforms such as Illumina. However, the short reads generated by these platforms limit specificity of taxonomic assignment and result in highly fragmented assemblies. Single molecule sequencing platforms are able to sequence much longer molecules and the output of these platforms, particular the PromethION from Oxford Nanopore now supports the study of complex microbial communities using shotgun metagenomics.

We assessed a variety of methods using both a ten-species mock community and clinical samples seeking to find a method capable of generating ultra-long reads (>100 kb) from these sample types. The results presented here demonstrate the importance of having lysis methods capable of extracting high-molecular weight DNA from recalcitrant organisms such as Gram-positive bacteria and fungi. Development of these methods is critical to support the growing field of clinical microbiome research including the ability to perform strain tracking and produce high-quality metagenome assembled genomes (MAGs) from metagenomic samples.

 

Learning Objectives:

  • Explain how to utilize mock microbial community standards to benchmark and optimize DNA extraction methods.
  • Explain the effects that different lysis methods have on long-read metagneomic sequencing.
  • Explain how a hybrid lysis technique can improve long-read data quality.

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
JUN 22, 2021 10:00 AM PDT
C.E. CREDITS
JUN 22, 2021 10:00 AM PDT
Date: June 22, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Antimicrobial resistance (AMR) has emerged as one of the principal public health problems of the 21st century. It threatens the effectiv...
OCT 15, 2019 10:00 AM PDT

The 'Three Peak Challenge' for long-read, ultra-deep stool metagenomics on the PromethION

Sponsored by: Zymo Research

Specialty

Molecular Biology

Microbiology

Genomics

Dna Sequencing

Biotechnology

Molecular Diagnostics

Genetics

Bioinformatics

Dna

Pcr/rt-Pcr/real-Time Pcr

Gene Expression

Cancer Research

Gene Sequencing

Cell Biology

Infectious Disease

Geography

North America52%

Europe23%

Asia12%

South America4%

Africa4%

Oceania3%

Registration Source

Website Visitors71%

Email Promotions23%

WAVE Newsletter Promotion4%

Trending Newsletter Promotion1%

Other1%

Job Title

Research Scientist18%

Student14%

Educator/Faculty11%

Executive10%

Scientist8%

Facility/Department Manager8%

Post Doc6%

Medical Laboratory Technician6%

Lab Management5%

Clinical Laboratory Scientist4%

Medical Doctor/Specialist4%

Microbiologist3%

Biologist2%

Organization

Academic Institution36%

Biotech Company8%

Government7%

Research Institute7%

Clinical Laboratory6%

Hospital5%

Government/public4%

Non-Profit Organization3%

Life Science Company3%

Distribution/sales2%

Medical School2%

Pharmaceutical Company2%

Diagnostic Company2%

Medical Center1%

Other3%


Show Resources
Loading Comments...
Show Resources