Cancer research is being revolutionized by targeted gene panels, whole exome sequencing (WES), whole genome sequencing (WGS), and transcriptome sequencing (RNA-Seq). Many analyses, such as comparison of tumor and normal cells, include comparison of variant calls. In this webinar, we will illustrate the benefits of leveraging read mappings in the comparison of WES and RNA-seq data using CLC Cancer Research Workbench. The CLC platform provides a number of tools, together building up a workflow, for analyzing WES and RNA-seq reads and for comparing the results. From this webinar you will learn how to analyse and compare variants from two patients using built-in workflows in CLC Cancer Research Workbench; Analysis steps include filtering results for tumor-specific variants, identifying variants from both WES and RNA-seq data, searching RNA-seq read mappings for variants previously called from exome data, and recovering previously detected variants in the WES data directly from the RNA-seq read mappings.