APR 06, 2022 9:00 AM PDT

Applications of multiplex digital PCR: Monitoring wastewater for SARS-CoV-2 and measuring Bacterial Gene Expression

Sponsored by: Thermo Fisher Scientific
Speaker

Abstract
Date:  April 06, 2022
Time: 9:00am (PDT),  12:00pm (EDT), 6:00pm (CEDT)
 
Digital PCR is a specialized tool for nucleic acid quantification, which has applications in many research areas including those in microbiology and virology research. Leveraging optical multiplexing, multiple targets expected within a single sample can be tested in a single digital PCR reaction, providing absolute quantification without the need for a reference or standard curve.
 
In this webinar, invited speaker Dr. Joan L. Slonczewski of Kenyon College will describe their ongoing research, which leverages digital PCR.
 
Highlights include:
  1. The use of the Applied Biosystems QuantStudio Absolute Q Digital PCR System* alongside the Combinati SARS-CoV-2 Wastewater Surveillance kit to monitor and measure the quantity of SARS-CoV-2 viral RNA from the wastewater influent of a village community including college residence halls—equating to approximately 2,500 village residents overall.
  2. The development and use of a custom quadruplex assay to measure bacterial mRNA expression of multidrug resistance pumps in E. coli.
* For Research Use Only. Not for use in diagnostic procedures.
 
Learning Objectives
  • Identify the benefits of using digital PCR for precise nucleic acid target measurement
  • Describe how multiplex digital PCR can be used as a tool for microbiology and virology research
  • Recognize the benefits of precise environmental surveillance for emerging pathogen research
 
 
Webinars will be available for unlimited on-demand viewing after live event.

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