OCT 30, 2024 11:00 AM PDT

Accelerating insights into changes in binding affinity of biomolecular charge variants using a simple icIEF fractionation and SPR workflow

Sponsored by: Nicoya Lifesciences
Speakers

Event Date & Time
Date: October 30, 2024
Time: 11:00 AM (PST), 2:00 PM (EST), 8:00 PM (CET)
Abstract
Charge heterogeneity in monoclonal antibodies can negatively impact binding affinity and potency, so it is critical to ensure that individual charge variants of biotherapeutic molecules are thoroughly characterized to ensure product quality. Dr. Chris Heger (Bio-Techne) and Dr. Mike Piazza (Nicoya Lifesciences) will illustrate a fast, simple icIEF fractionation and digital SPR workflow that provides in-depth characterization of the charge variants of a therapeutic bispecific antibody (BsAb) Mosunetuzumab and a research-grade biosimilar. Using a combination of the MauriceFlex™ system and Alto™ Digital SPR, they demonstrate a novel method that utilizes imaged capillary isoelectric focusing (icIEF)-based charge separation and fractionation followed by binding affinity analysis using surface plasmon resonance, offering a robust and efficient approach to evaluating charge variants. Prior LC-MS analysis of the fractions uncovered interesting differences between the two molecules. The speakers will show how the high-purity fractions of both the innovator drug and a research-grade biosimilar were analyzed by SPR for binding studies with their ligands CD3 and CD20, revealing notable differences in binding affinity, particularly between the biosimilar’s acidic peak and ligand CD20.
 
Learning Objectives
  • How to set up a simplified workflow that uses (icIEF)-based charge separation and fractionation followed by digital SPR for deeper insights into charge variant binding affinity.
  • How leveraging these two benchtop platforms can uncover critical differences between a therapeutic mAb and its biosimilar.
  • What additional context can be gained on these charge variant differences using mass spectrometry analysis.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 

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