SEP 05, 2019 12:00 PM PDT

Advancing astrocyte research with novel tools for characterization, isolation, and cultivation

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • R&D Project Manager Neuroscience - Miltenyi Biotec
    Biography
      Melanie Jungblut, Ph.D., studied biology and chemistry at the University of Kaiserslautern, Germany. She received her PhD in Neurobiology/Biotechnology at the Max Planck Institute for Polymer Research and University of Mainz, Germany. In 2007 she joined Miltenyi Biotec as a postdoc and is group leader in the R&D for product development in the field of neuroscience since 2011.

    Abstract

    DATE: September 5, 2019
    TIME: 12:00pm PDT

    Astrocytes are a major cell type in the mammalian CNS. They are involved in a number of essential processes such as synapse formation and function, as well as blood-brain barrier formation and control of cerebral blood flow. Furthermore, it has been shown that astroglial cells act as neural stem cells (NSCs) in the neurogenic regions of the adult brain. However, still many aspects of the molecular mechanisms underlying the role of astrocyte in development and diseases are unknown, which is at least partly due to the lack of tools available for astrocyte isolation and characterization of different astrocyte subtypes. In this webinar we will describe an optimized procedure for dissociation of neonatal as well as adult mouse brain and introduce new antibodies and technologies for the characterization and isolation of living astrocytes as well as NSCs.  As a single cell suspension with high cell viability and a minimum of cell debris is the prerequisite for successful downstream processing, we present an optimized automated dissociation procedure for neonatal as well as adult mouse brain that yields a large number of pure viable cells. Furthermore, we demonstrate different tools and techniques for isolation and cultivation of astrocytes as well as neural stem cells. Whereas neonatal and adult astrocytes are isolated by magnetic cells sorting, sorting of adult NSCs is carried out using the MACSQuant® Tyto®, a novel multiparametric cell sorting device that uses a microchip-based technology for sterile and gentle cell isolation. In summary, the webinar gives an overview on novel approaches for the characterization and isolation of astrocytes and NSCs by combination of optimized cell preparation methods and sophisticated cell sorting technologies.

     

    Learning Objectives:

    • Unique astrocyte specific antibodies for characterization and isolation of viable astrocytes.
    • Novel approach for gentle isolation of pure adult neural stem cells (NSCs) using the MACSQuant® Tyto® technology.
    • Novel technologies for optimized neonatal and adult brain tissue dissociation.
    • Improved serum-free culture of primary astrocytes and adult neural stem cell.

     

    Webinars will be available for unlimited on-demand viewing after live event.

     

    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

     


    Show Resources
    You May Also Like
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    APR 07, 2020 8:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    MAR 03, 2020 9:00 AM JST
    C.E. CREDITS
    MAR 03, 2020 9:00 AM JST
    DATE: March 3, 2020 TIME: 9:00am JST A major limitation in the ex vivo expansion of harvested human hematopoietic stem-progenitor cells (HSPCs) is the rapid differentiation of HSPCs at the e...
    FEB 25, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 25, 2020 9:00 AM PST
    Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
    Loading Comments...
    Show Resources