JAN 14, 2020 4:00 PM PST

Advancing Biomedical Science: Live Cell Imaging of Vesicle Trafficking using perfectly balanced Super-resolution Dragonfly Microscopy

Sponsored by: Andor
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Product Specialist - Life Sciences, Andor Technology
    Biography
      Claudia graduated in Applied Chemistry, has a MSc in Human Molecular Biology, a PhD in Cell Biology, and two postdocs in Cell Biology.
      With more than 15 years' experience in research, Claudia has worked in several areas like cell biology, HIV studies, oncobiology, endocrinology and neurosciences. In 2010 Claudia joined Universidade do Algarve as a facility manager and three years after Claudia become the director of Advanced Light Microscopy Facility.
      In May 2019, Claudia joined Andor Technologies as a Product Specialist in the Microscopy Systems Division.
    • Staff Researcher, Department of Physiology, Kanazawa University
      Biography
        Dr Kazuaki Yoshioka is a staff researcher at Department of Physiology (Prof. Yoh Takuwa's Lab.), Kanazawa University Graduate School of Medical Science, in Japan.

        Dr Kazuaki Yoshioka did his PhD at Tokyo University of Agriculture and Technology in the lab of Prof. Hideaki Matsuoka. Further, he undertook a postdoctoral researcher role at Bioanalytical Systems Inc. (BASi) in the USA; he then moved for a second postdoctoral researcher position at Tokyo Metropolitan Institute of Medical Science. Currently, Dr Yoshioka research focus is in the field of vesicle trafficking.

        Dr Yoshioka aims to understand the roles of PI3 kinases in vesicle trafficking, as well as their importance in normal homeostasis and disease. Dr Yoshioka, has an outstanding publication track record, with an H-index of 27 given from 57 publications in the web of science that were cited 1862 times.

        Following Dr Yoshioka interesting work on vesicle trafficking, we wanted to understand what motivates his vesicle trafficking research and what are the challenges that he needs to overcome to be able to answer his research questions. Here, we present an interview on Dr. Yoshioka's recent challenges and discoveries.
      • Product and Business Manager, Imaris
        Biography
          Meredith is the Imaris Product and Business Manager. She previously worked as an Imaris Sales Engineer from 2010-2017. Meredith received a MS in Biology from the University of Oregon and studied cell signaling and tissue polarity in Mark Peifer's lab at the University of North Carolina at Chapel Hill.

        Abstract
        Webinars will be available for unlimited on-demand viewing after live event.  Questions can be submitted during the on-demand period and will be answered via the contact information you provided at the time of registration.
         
        Live Event will broadcast: January 15, 2020, 9:00am JST, January 14, 2020, 4:00pm PST, 7:00pm EST
         
        Membrane trafficking, i.e. endocytic/exocytic, endo-lysosomal and autophagic, pathways are well studied, this field has grown rapidly over the last decade. Recently a “new era of membrane trafficking diseases” as emerged and de-regulation of vesicle trafficking pathways was shown to be related to syndromes such as: Parkinson’s, Alzheimer’s, cancer, and metabolic syndromes like type2-diabetes.

        Intracellular vesicles (e.g. endosomes and lysosomes) with sizes of a few hundred nanometres cannot be resolved by conventional confocal microscopy. Super-resolution microscopy techniques like PALM and STORM are slower than conventional live-imaging technologies and /or require high laser power, rendering these approaches unsuitable for super-resolved live imaging.

        We will present actual membrane trafficking events captured using Andor’s Super-Resolution Radial Fluctuation (SRRF-stream) implemented via the highly sensitive iXon camera. Andor´s imaging technology offered new insights on the role of PI3-kinase in clathrin-mediated pinocytosis. Understanding this fundamental mechanism of vesicle trafficking could have implications for many diseases and might offer new therapeutic targets.
         
        Learning Objectives:
        • Identify the difficulties in vesicle imaging.
        • Discover how to image sub-resolution vesicle trafficking events.
        • Discuss the role of Pi3-kinase in vesicle endocytosis.
        • Use new tools to analyse vesicle trafficking and interaction in 3D
         
         
        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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