Advancing mRNA Therapeutics with Mass Photometry

mRNA technologies have rapidly evolved into one of the most exciting and versatile classes of modern medicines, powering breakthroughs in vaccines and emerging therapies for metabolic, cardiovascular, and oncology applications. But as the field accelerates, so does the need for deeper insight into the quality and structural integrity of mRNA products.

Throughout manufacturing, purification, formulation, and storage, mRNA molecules can develop impurities such as double stranded RNA, truncated transcripts, and higher order multimers. These species can reduce translational efficiency, hinder effective lipid nanoparticle encapsulation, and ultimately compromise product safety and performance. Yet many conventional analytical tools rely on denaturing conditions, making it difficult to detect and quantify these structures in their native state.

In this webinar, Mark Dickman, Professor at the University of Sheffield, explores how mass photometry, a label free, single molecule analytical technology, is reshaping mRNA characterization. Professor Dickman will highlight how combining non‑denaturing ion‑pair reversed phase HPLC (IP RP HPLC) with mass photometry enables precise measurement of mRNA monomers and multimers directly in solution—providing accurate insights into size, purity, and aggregation under native conditions.

Join us to learn how this innovative approach can enhance your ability to assess critical quality attributes and support the development of safer, more effective mRNA based therapeutics.

Learning Objectives:

• Develop a clear understanding of mRNA‑based medicines and the critical quality attributes that shape their safety, efficacy, and overall performance.

• Examine the major analytical challenges involved in evaluating mRNA integrity, purity, and structural complexity across development and manufacturing workflows.

• Discover how non‑denaturing IP‑RP‑HPLC used in combination with mass photometry offers a robust, complementary methodology for characterizing mRNA monomers and multimers under native conditions.