JUN 08, 2021 8:00 AM PDT

Antigen-specific CD4 T cell responses in humans: SARS-CoV-2 and beyond

Sponsored by: Miltenyi Biotec
Speakers

Abstract

Date:  June 08, 2021

Time: 8:00am PDT, 11:00am EDT, 5:00pm CEST

 

COVID-19 displays high clinical variability, but what determines its severity is unclear. Analysis of antigen-specific T cell responses has contributed to our understanding of the induced immune reaction, and is important in characterizing and potentially predicting the success of vaccination and clinical outcome. Pre-existing T cell memory generated by frequent infections with the related “common cold” Coronavirus (CCCoV) has been hypothesized as a protective mechanism. Prof. Scheffold and his colleagues used a sensitive technology to characterize antigen-reactive T cells directly ex vivo, in combination with closed-system cell sorting on the MACSQuant® Tyto® to enrich and purify antigen-reactive T cells. They were able to characterize SARS-CoV-2‒ and CCCoV-specific T cells from healthy donors and COVID-19 patients using single cell gene expression profiling. Moreover, multiparameter flow cytometry, TCR avidity, and cross-reactivity measurements were used to further deepen the characterization of these cells. The data suggest that pre-existing SARS-CoV-2‒specific memory is not only not protective, but may in fact contribute to the development of severe COVID-19.

 

Learning Objectives

  • What are the latest insights in the role of SARS-CoV-2‒ and CCCoV-specific T cells in COVID-19 protection and disease progression?
  • Which technologies enable efficient, gentle, and safe isolation of antigen-specific T cells for subsequent deep profiling by, e.g., single-cell genomics analyses?

 

Webinars will be available for unlimited on-demand viewing after live event.


Show Resources
You May Also Like
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
AUG 16, 2022 11:00 AM PDT
AUG 16, 2022 11:00 AM PDT
Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
JUN 01, 2022 9:00 AM PDT
JUN 01, 2022 9:00 AM PDT
Date: June 01, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The manufacturing process for cell and gene therapies is complex and can contain process-related impurities from multiple...
Loading Comments...
Show Resources