MAY 19, 2020 10:00 AM EDT | APAC MAY 20, 2020 1:00 PM CST

Applications in Biomarker Discovery and CAR T Therapy Using High-plex RNA & Protein Spatial Profiling on FFPE Tissue

Speaker
  • Director of the Center for Cellular Immunotherapies, Perelman School of Medicine and Director of the Parker Institute for Cancer Immunotherapy, University of Pennsylvania
    Biography
      Carl June, MD, is the Richard W. Vague Professor in Immunotherapy in the Department of Pathology and Laboratory Medicine. He is currently director of the Center for Cellular Immunotherapies and director of Translational Research in the Abramson Cancer Center of the University of Pennsylvania, and is an investigator of the Abramson Family Cancer Research Institute. He is a graduate of the Naval Academy in Annapolis and Baylor College of Medicine in Houston, 1979. He had graduate training in immunology and malaria with Dr. Paul-Henri Lambert at the World Health Organization, Geneva, Switzerland in 1978 and 1979, and post-doctoral training in transplantation biology with E. Donnell Thomas and John Hansen at the Fred Hutchinson Cancer Research Center in Seattle from 1983 to 1986. He is board certified in Internal Medicine and Medical Oncology. He maintains a research laboratory that studies various mechanisms of lymphocyte activation that relate to immune tolerance and adoptive immunotherapy for cancer and chronic infection. In 2011, his research team published findings, which represented the first successful and sustained demonstration of the use of gene transfer therapy to treat cancer. Clinical trials utilizing this approach, in which patients are treated with genetically engineered versions of their own T-cells, are now underway for adults with chronic lymphocytic leukemia and adults and children with acute lymphoblastic leukemia. Early results in that group show that 90 percent of patients respond to the therapy, and more recently, trials of this approach have begun for patients with other blood cancers and solid tumors including pancreatic cancer, mesothelioma and the brain cancer glioblastoma. In 2014, it became the first personalized cellular therapy for the treatment of cancer therapy to receive the U.S. Food and Drug Administration's prestigious Breakthrough Therapy designation.

    Abstract

    The GeoMx Digital Spatial Profiling technology enables high-plex RNA and protein profiling of any region within any tissue type. This workshop will highlight the latest developments toward enabling spatial transcriptome analysis as well as highlighting novel applications in CAR T biology. Dr. Carl June, MD (University of Pennsylvania), will describe this application in a patient undergoing CAR T cellular therapy. GeoMx DSP was used to spatially analyze thousands of RNA targets to understand differences in tumor-related pathways in the presence of CAR T cells and the exhaustion status of the CAR T cells themselves.

    GeoMx DSP is for Research Use Only and not for use in diagnostic procedures.


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