Approaches to sample preparation

Speaker

Abstract
Even as next-generation sequencing (NGS) has become widely used, and the costs have been reduced, it remains critical to achieve reliable and robust data at the end of the sequencing run. Sequencing labs have very little control over the quantity and quality of the sample received from the pathology lab or clinic, yet sample quality can have a major impact on the downstream processes.
 
While the manual, column-based approach to nucleic acid isolation is still widely used, scientists under pressure from high-throughput demands are turning their attention to superparamagnetic beads. These are automation friendly and well-suited for a range of applications, including sample preparation for next generation sequencing (NGS). Each technique has pros and cons, and selection will depend on sample type, throughput requirements, targets, and other factors.
 
In this webinar you will:
  • Learn how to check sample quality against set criteria and assess the potential impact on data outcomes in relation to genomic and prenatal testing applications
  • Review modifications to workflows, protocol steps and NGS analysis to manage the quality of complex samples such as FFPE and liquid biopsy
  • Understand the benefits and drawbacks of different approaches to nucleic acid isolation with particular focus on column-based and magnetic bead-based approaches
  • Compare the benefits and drawbacks of different approaches to nucleic acid isolation with particular focus on column-based and magnetic bead-based approaches
 

Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
OCT 12, 2021 9:00 AM PDT
C.E. CREDITS
OCT 12, 2021 9:00 AM PDT
Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
MAY 18, 2021 8:00 AM PDT
C.E. CREDITS
MAY 18, 2021 8:00 AM PDT
Date: May 18, 2021 Time: 8:00am (PST) The global pandemic has caused an increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provide qu...
Loading Comments...
Show Resources