DEC 13, 2022 8:00 AM PST

Assessing CRISPR On-Target Editing and Structural Changes with UDiTaS™ using Tagify™ Reagents

Sponsored by: seqWell
Speakers

Abstract
Date:  December 13, 2022
Time: 8:00am (PST),  11:00am (EST), 5:00pm (CET)
 
Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. We developed a Uni-Directional Targeted Sequencing methodology, UDiTaSTM, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion events (indels), all in a single reaction. Editas processes thousands of UDiTaS reactions yearly, however producing a large-scale supply of a Tn5 transposase complexes (enzyme complexed with next generation sequencing adapters) to create UDiTaS sequencing libraries has been challenging. seqWell has provided the solution with their Tn5 TagifyTM toolkit to generate custom Tn5-adpater complexes to meet our demands. Custom barcoded adapters are provided to seqWell, and optimized, ready-to-use Tn5 transposase/adapter complexes are created and returned. Custom Tagify batches are reproducible allowing us to continue processing thousands of reactions yearly with consistent results.
 
Learning Objectives
  • Understanding the power of UDiTaS to measure structural changes resulting from CRISPR-Cas9 editing
  • Ready-to-use custom UDiTaS reagents are generated using the Tagify toolkit
  • Consistent custom Tagify batches for reproductible results
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

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