FEB 04, 2021 8:00 AM PST

Automation of organoid assays requiring a 3D Matrix with dragonfly® discovery

Sponsored by: SPT Labtech
Speaker
  • Senior Application Scientist, Corning Life Sciences
    Biography
      Hilary Sherman is a Senior Scientist in the Corning Life Sciences Applications Lab located in Kennebunk, ME. Hilary has been with Corning Incorporated since 2005 and has worked with a wide variety of cell types including mammalian, insect, primary, stem cells and organoids in a vast array of applications. Her key roles at Corning involve creating technical documents such as protocols and whitepapers as well as providing technical support and training for both the Corning sales force and customers. Hilary received her B.S. degree in Biology from the University of New Hampshire. In the last several years, Hilary has focused on 3D cell culture applications including human organoid culture.

    Abstract
    Date:  February 4, 2021
    Time: 8:00am (PST),  11:00am (EST)
     
    Over the last several years, there has been increased interest around the use of patient derived organoids for personalized medicine and drug discovery. The unique ability of organoids to recapitulate the organ structure, model disease, and maintain the genetic diversity of the donor tissue makes them a promising tool. In order to reach their full research potential, organoids need to be amenable to more automated and high throughput methods. Automating the handling of organoids has been problematic due to the required manipulation of viscous basement membrane extracts. Here, we demonstrate the use of the SPT Labtech dragonfly® discovery to accurately and evenly dispense small (3 µL) droplets of human intestinal organoids mixed with Corning® Matrigel® matrix for organoid culture, followed by a forskolin induced swelling (FIS) assay
     
    Learning Objectives
    • Learn about a novel method to automate organoid assays that require ECMs
    • See a demonstration of how organoids could be used for personalized medicine through a forskolin induced swelling assay with intestinal organoids
     
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