Beyond conventional: Using advanced spectral solutions to expand single cell and population analysis

As the fields of flow cytometry and spatial biology advance, researchers are uncovering exciting applications for the expanding data from their samples. Innovative reagents, fluidic and optical engineering, and advanced detection technologies significantly boost data speed, accuracy, and depth. Challenges persist, particularly in analyzing rare, clog-prone samples and deconvoluting fluorophore signals. This seminar will showcase data from two new spectral platforms and offer tips for maximizing their capabilities in advanced immunology and oncology research.

 

The spectral-enabled Invitrogen™ Attune™ Xenith™ Flow Cytometer, with acoustic focusing technology, advanced fluidics sensing, and UV to NIR detection helps provide deeper insights into even highly concentrated, high aggregate samples. Its software facilitates switching between conventional compensation and spectral unmixing, offering spillover guidance for each workflow. The Invitrogen™ EVOS™ S1000 Spatial Imaging System streamlines whole slide tissue imaging, enabling rapid 9-plex detection by means of integrated spectral unmixing.

 

We will address challenges in difficult sample types and offer solutions for overcoming common hurdles, with seminar outcomes including:

 

• Understanding the impact of acoustic focusing on flow cytometric analysis.
• Discovering how clog-resistant design maintains workflow efficiency and prevents poor-quality acquisitions.
• Gaining insights into spectral workflows, maximizing panel complexity and acquisition efficiency with reduced-spillover fluorophores and optimized sample preparation.

Who should Attend:

• Immunologists working on cell sorting, immune profiling, and flow cytometry
• Oncologists focusing on cancer research and tumor immunology
• Neuroscientists advancing brain spatial analysis and multiplex imaging techniques
• Core lab researchers looking to enhance their workflows with advanced technologies
• Anyone interested in pushing the boundaries of cellular analysis with spectral capabilities