Beyond Sequencing: Population Analysis in an Era of Mass Spectrometry Proteomics

DATE: December 14, 2016
TIME: 9:00 AM PT, 12:00 PM ET

Microarrays and nucleotide sequencers have become commonplace tools in current research, yet until now, equivalent technologies for providing measurements of the proteome and metabolome have remained elusive. Recent developments in mass spectrometry (MS)-based proteomics, including SWATH-MS, are now permitting reliable, high-speed, and accurate quantification of thousands of proteins across hundreds of samples. The disparities between mRNA and protein regulation have long been known, but only now can we reliably quantify the proteome’s complementary perspective on cellular mechanics. We have applied SWATH-MS, along with transcriptomics, across a diverse population to uncover new insights on mitochondrial protein localization and the stoichiometry of oxidative phosphorylation.
 
Learning Objective 1: See how proteomics can help us understand natural genetic variation across populations 

Learning Objective 2: Understand why proteomics is now a viable addition to DNA and RNA sequencing projects 

Learning Objective 3: Consider proteomics as a mature technology, ready for mainstream biologists