DATE: March 24, 2016
TIME: 10am Pacific time, 1pm Eastern time
Protein-protein interactions form a network whose structure drives cellular function and whose organization informs all biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions, 86% unknown, among 7,668 proteins. BioPlex accurately depicts known complexes, attaining 80-100% coverage for most CORUM complexes. Network structure reveals 4 key features: 1)
BioPlex subdivides into >300 communities, uniting proteins with shared function. 2)
Interactions predict 2,968 associations among co-occurring Pfam domains. 3)
Attributes including localization, biological process, and molecular function were determined for thousands of proteins - many uncharacterized. 4)
BioPlex reveals interactions of biological or clinical significance. To demonstrate complementary studies inspired by BioPlex, we interrogated interactions of wild-type and mutant VAPB variants implicated in familial Amyotrophic Lateral Sclerosis. The network provides a framework for hypothesis generation and refinement as applied to protein function, mechanism, and activity.
- Learn how affinity purifications can be performed at scale and the associated caveats.
- Understand how an interaction network can be used to predict a protein’s cellular properties.