Date: September 20, 2022
Time: 9:00am PDT, 12:00pm EDT, 6:00pm CEST
Date: September 21, 2022
Time: 9:00am SGT
Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of interest and reverse-transcribed into complementary DNA (cDNA). The levels of gene expression are then determined from the quantity of cDNA amplified by PCR. The initial stages of the RT-qPCR workflow, such as RNA isolation and reverse transcription, can critically impact the accuracy of the quantitation of gene expression.
- Important considerations for RNA isolation
- Tools for efficient cDNA preparation
- Sources of poor RT-qPCR results
- Tips for overcoming challenges, for highest success
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