JUN 17, 2020 11:00 AM PDT

Breaking the Bonds, Not the Bank: New Possibilities with e-MSion's ExD Cell

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Abstract

The ExD Option, available for both new and existing Agilent instruments, offers superior electron fragmentation in combination with collision induced dissociation (CID) compared to other existing fragmentation technologies. It allows for the far more complete characterization of proteins and glycoproteins with unprecedented accuracy, speed and simplicity. Unlike ETD, the ExD technology can produce higher energy electrons to fragment across more complex ring structures to give better characterization of glycans as well as to provide better mapping of disulfide chains. The ExD technology preserves labile post-translational modifications on large peptides and intact proteins, which allows for the simultaneous quantitation of multiple phosphorylation and glycation sites in bottom-up and middle down proteomic work flows.In addition, ExD induces side chain fragmentation that can differentiate isobaric leucine from isoleucine, isoaspartate from aspartate and other degradation products affecting biopharmaceutical quality. The superior dissociation of native proteins by ExD with retention of post-translational modifications and side chain differentiation opens new opportunities for more complete characterization of complex proteoforms in a highly cost-effective platform.

For Research Use Only. Not for use in diagnostic procedures.


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