MAY 10, 2017 6:00 AM PDT

CATS - a novel library preparation method for ultrasensitive deep sequencing of extracellular circulating RNA and DNA

Sponsored by: Diagenode
C.E. Credits: CEU
Speaker
  • Postdoctoral Scientist, German Cancer Research Center, Heidelberg
    Biography
      Andrey Turchinovich received his PhD in Neuroscience at Ruhr-University Bochum, where his research was concentrated on RNA interference and functional analysis of genes involved in neurodegeneration. He is currently working as a postdoctoral scientist at the German Cancer Research Center in Heidelberg. His research is focused on developing new methods for early diagnosis of tumors based on extracellular circulating RNA and DNA. Among his major achievements is developing a novel DNA library preparation method which allows deep sequencing of ultra-low inputs of nucleic acids and detection of circulating DNA and RNA in bio-fluids with unprecedented sensitivity. Furthermore, together with his colleagues, he has previously explained the origin and the stability of extracellular circulating microRNAs.

    Abstract

    Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of nucleic acids still remains a major challenge, especially for degraded nucleic acids like circulating DNA or RNA. This, together with high costs and time requirements, impedes many important applications of MPS in medicine and fundamental science. We have developed the “Capture and Amplification by Tailing and Switching” (CATS) method to generate ready-to-sequence DNA libraries from picogram amounts of either DNA or RNA molecules in a time frame of several hours, that permits sequencing of circulating nucleic from diagnostic volumes of liquid biopsies with unprecedented depth. CATS approach could find broad application in diverse research areas such as translational medicine including therapy monitoring, prediction, prognosis and early detection of various human disorders, and will permit high-throughput DNA sequencing from previously inaccessible material such as minute forensic and archeological samples.


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