AUG 11, 2020 10:00 AM PDT

Better resolution for CE-SDS analysis of new biopharmaceutical modalities through fine-tuning the separation temperature

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Applications Manager, SCIEX Separations
    Biography
      Recipient of the 2016 BioProcess International Best Technology Application - Analytical Award for his work on SCIEX Fast Glycan Technology, András Guttman currently leads the application efforts at Sciex Separations (Brea, CA) and holds the Translational Glycomics Professorship at the Horváth Csaba Laboratory of Bioseparation Sciences (University of Debrecen, Hungary). His work focuses on capillary electrophoresis and CE-MS based proteomics and glycomics analysis of biopharmaceutical, biomedical and cell biology interests. Dr Guttman previously held academic appointments at Northeastern University (Boston, MA) and a Marie Curie Chair at University of Innsbruck (Austria) as well as industrial positions at Novartis (La Jolla, CA), Genetic BioSystems (San Diego, CA), and Beckman Instruments (Fullerton, CA), developing high resolution capillary electrophoresis and microfluidics based separation methods. Professor Guttman has more than 269 scientific publications, wrote 35 book chapters, edited 4 textbooks and holds 19 patents. Until recently he served on the CASSS Board and as president of the Hungarian Chapter of the American Chemical Society. He is on the editorial boards of numerous international scientific journals. Dr Guttman graduated from University of Veszprem, Hungary in chemical engineering, where he also received his doctoral degree. He was recognized by the Analytical Chemistry Award of the Hungarian Chemical Society in 2000, elected as a member of the Hungarian Academy of Sciences in 2004, named as Fulbright Scholar in 2012, received the CASSS CE Pharm Award in 2013, the Arany Janos medal of the Hungarian Academy of Sciences, the Pro Scientia award of the University and the Dennis Gabor Award of the Novofer Foundation in 2014. Dr. Guttman is also the recipient of the 2017 Dal Nogare Award of the Delaware Valley Chromatography Forum, which he received at Pittcon earlier this year.

    Abstract
    DATE:  August 11, 2020
    TIME:   10:00am PDT, 1:00pm EDT
     
    Biopharma researchers facing a difficult time adopting CE-SDS for purity/heterogeneity analysis of new protein modalities can now rapidly accommodate these analytes by simple, precise temperature adjustments to achieve the desired resolution. Since the electromigration property of any protein – SDS complex is an activated process and each and every single molecule have their own activation energy requirement, one can adjust the separation temperature to alter the separation performance for different samples. In other words, the separation between any proteins of interest can be easily fine-tuned by precisely varying the separation temperature in the PA 800 Plus Pharmaceutical Analysis System to attain the required resolution. SDS-CGE analysis of a biotherapeutics revealed no correlation between the activation energy requirement of the different species and their molecular weights, further emphasizing the importance of separation temperature optimization to obtain high resolution between the solute molecules of interest. 
     
    Learning Objectives:
    • Understand how temperature effects the separation resolution of CE-SDS methods for varying analytes
    • Easily optimize the separation of protein therapeutics by fine tuning the capillary temperature
    • Utilize the patented circulating liquid cooling technology allowing you to reproducibly adjust the separation temperature
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

     

     


    Show Resources
    You May Also Like
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    NOV 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    SEP 02, 2020 7:00 AM PDT
    C.E. CREDITS
    SEP 02, 2020 7:00 AM PDT
    DATE: September 2, 2020 TIME: 03:00pm PDT, 6:00pm EDT Spatial omics is an expanding collection of methods to examine biological molecules in their geographical context. By retaining the prec...
    Loading Comments...
    Show Resources
    Attendees
    • See more