JUN 17, 2021 7:00 AM PDT

Cell DIVE: a robust platform for multiplexed whole slide imaging and single-cell analysis

Sponsored by: Leica Microsystems
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
DATE:  June 17, 2021
TIME:  7:00am (PDT),  10:00am (EDT)
 
Colorectal cancer is a particularly challenging disease for immunotherapy given tumor heterogeneity. Multiplexed whole slide imaging allows deep contextual analysis of cancer tissue and provides new insights into cancer tissue heterogeneity, including cell-to-cell interactions and the tumor immune microenvironment. In the current study, the Cell DIVE™ multiplexed immunofluorescence solution was used to comprehensively map 36 biomarkers on a single Colon Cancer Tumor Microarray (TMA), with over 96 tissue core samples, enabling multiplexed biomarker labeling of tumors from multiple patients. Stitched images were imported into HALO® image analysis software (www.indicalab.com). For analysis, a subset of 18 biomarkers were used for tissue classification, cell segmentation, cell phenotyping and spatial analysis. Multiplexed tissue immunofluorescence imaging of a multi-patient TMA provides unprecedented and novel insights into cell populations and spatial interactions in the tumor microenvironment across patient samples.
 
Learning Objectives:
 
  • Identify why immunotherapy in colon cancer is challenging.
  • Explain how multiplexed imaging provides new insights into tumor heterogeneity, enabling tumor stratification, that may help define new markers for future therapy
  • Examine single cell analysis of multiplexed immunofluorescence Cell DIVE™ images of biomarkers on a single TMA slide arrayed with 96 tissue samples
  • Explore how precise image processing between staining rounds allows the exploration of biomarker co-expression or phenotypes in single cells across all biomarkers
 
 
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