NOV 13, 2018 03:00 PM CET

From cell seeding to analysis - Getting the best out of your cell-based assay

SPONSORED BY: Eppendorf
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Application Specialist Cell Handling, Eppendorf AG
    Biography
      Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.
    • Application Specialist, Promega GmbH
      Biography
        Mr. Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.

      Abstract:
      DATE: November 13,2018
      TIME:  3:00pm CET, 7:30pm IST, 06:00am PST, 09:00am EST
       
      The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite of choosing the appropriate assay type, there are many more factors to be considered to decrease data variability.
       
      In this webinar Eppendorf and Promega are joining forces to optimize your assay results. We will have a look at the complete cell culture workflow and discuss measures you can take to get the best out of your cell-based assay. Furthermore, practical tips and tricks to avoid common pitfalls will be discussed with one of our most experienced Cell Culture Application Specialists.
       
      In this webinar you will learn:
      • How to find the best time point for analysis
      • What are the pros/cons of the different modes of detection?
      • Tips and tricks for optimal cell culture routine and assay setup
      • Tips and tricks for cell-based assay setup

      Show Resources
      You May Also Like
      APR 27, 2018 10:00 AM PDT
      C.E. CREDITS
      APR 27, 2018 10:00 AM PDT
      DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
      MAY 24, 2018 09:30 AM PDT
      C.E. CREDITS
      MAY 24, 2018 09:30 AM PDT
      DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
      MAY 03, 2018 11:00 AM PDT
      MAY 03, 2018 11:00 AM PDT
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      MAY 22, 2018 08:00 AM PDT
      C.E. CREDITS
      MAY 22, 2018 08:00 AM PDT
      DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
      JUN 29, 2018 09:00 AM PDT
      C.E. CREDITS
      JUN 29, 2018 09:00 AM PDT
      DATE: June 29, 2018TIME: 09:00AM PDT, 12:00PM EDT There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a r...
      MAY 02, 2018 08:00 AM PDT
      C.E. CREDITS
      MAY 02, 2018 08:00 AM PDT
      Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
      Loading Comments...