MAY 13, 2015 6:00 AM PDT

Challenges and New Solutions for Isolating Exosomes, Other Extracellular Vesicles and Vesicular RNAs

Speaker
  • Associate Director, Scientific Applications, Sample Technologies Development, Qiagen GmbH
    Biography
      Martin Schlumpberger has a Ph.D. in chemistry and more than 20 years of experience working in genetics and molecular biology. He did his postdoctoral fellowship at the Institute of Neurodegenerative diseases at UCSF. He has been at QIAGEN for over 13 years developing new strategies and products, both manual and automated, to isolate nucleic acids from a wide variety of samples, including extracellular RNA and miRNA from serum and plasma, exosomal RNA, as well as intact exosomes and other extracellular vesicles (EVs). He started as a research scientist and is currently an Associate Director in the Sample Technology Development group.

    Abstract

    A new affinity-based spin column procedure has been developed for highly specific isolation of exosomes and other extracellular vesicles (EVs) from serum or plasma, as well as for vesicular RNA content (exoEasy and exoRNeasy protocols). Results indicate that vesicular RNA (incl. miRNA) can be separated from non-vesicular RNA, and that fully intact long RNAs as well as miRNAs can be recovered and successfully profiled. In addition, the influence of different sample collection, handling and processing devices and workflows on RNA representation has been tested. Learning objectives: 1. Isolation of EVs and vesicular RNA from serum, plasma, and other biofluids 2. Detection and profiling of extracellular miRNAs and long RNAs 3. Challenges and solutions associated with sample collection and processing of biofluids for analysis of EVs and vesicular content


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