MAY 08, 2019 9:00 AM PDT

Circular Synthesized CRISPR/Cas gRNAs for Functional Interrogations in Single and Multiplex Formats

C.E. Credits: CEU P.A.C.E. CE Florida CE
Speaker
  • CSO, Vivlion
    Biography
      Dr. Manuel Kaulich studied biotechnology and molecular life science at the University of Lübeck and obtained his PhD in the laboratory of Erich A. Nigg at Max Planck Institute of Biochemistry in Martinsried (Germany) and the Biozentrum in Basel (Switzerland), respectively. In 2011 he moved to the group of Steven F. Dowdy at the University of California in San Diego, where he first became interested in gene editing technologies. Since December 2015, Manuel is an independent group leader at the Institute of Biochemistry II at Goethe University Frankfurt. Manuel's academic interest lies in cell cycle entry regulation in the context of malignancy. He also developed a strong interest in the CRISPR/Cas9 and rAAV gene editing technologies and has developed technologies to efficiently perform targeted gene replacement in human cells. He is the lead inventor of the 3Cs technology which forms the key of Vivlion's IP portfolio. Together with Ivan Dikic, Manuel Kaulich heads the Frankfurt CRISPR/Cas9 Screening Center (FCSC) at Institute of Biochemistry II. This academic center serves the local research community with technical and bioinformatical expertise to perform gene knockouts for functional studies as well as large scale genome-wide and hypothesis-driven CRISPR/Cas-based screenings.

    Abstract

    CRISPR/Cas gene editing has become the gold standard for individual gene perturbations as well as high-throughput functional screenings, with a rapidly increasing demand for high-quality CRISPR/Cas reagents requiring novel and innovative technologies to satisfy all user groups. The recently described 3Cs technology to generate bias-free gRNA and shRNA reagents addressees this issue and provides a robust and rapid, PCR- and cloning-free protocol for the generation of single and multiplexed gRNAs for functional interrogations. In my lecture, I will present selected single and multiplex gene editing applications to demonstrate the general applicability and power of 3Cs reagents.

    Learning Objectives: 

    1. What defines 3Cs gene editing reagents
    2. What are the current forms of 3Cs reagents


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