CRISPR/Cas gene editing has become the gold standard for individual gene perturbations as well as high-throughput functional screenings, with a rapidly increasing demand for high-quality CRISPR/Cas reagents requiring novel and innovative technologies to satisfy all user groups. The recently described 3Cs technology to generate bias-free gRNA and shRNA reagents addressees this issue and provides a robust and rapid, PCR- and cloning-free protocol for the generation of single and multiplexed gRNAs for functional interrogations. In my lecture, I will present selected single and multiplex gene editing applications to demonstrate the general applicability and power of 3Cs reagents.
1. What defines 3Cs gene editing reagents
2. What are the current forms of 3Cs reagents