MENU
JAN 12, 2016 8:00 AM PST

WEBINAR: Combined Use of Multiple Particle Characterization Technologies to Evaluate Targeted Liposomal Formulations: Implications for General Nanoparticle Development

Speaker

Abstract
January 12, 2016, 8:00am PT, 11:00am ET, 3:00pm GMT

Targeted nanoparticle applications have become a major focus of translational development of both diagnostics and therapeutics for clinical use.  One of the oldest and best established nanoparticle formulations is liposomes, which can be readily targeted to tissue markers by chemical conjugation to ligands such as antibodies.  The cardiovascular research group at UTHealth has been exploring molecular targeting strategies for our intrinsically echogenic liposomes (ELIP) for nearly 25 years.  As part of this effort, we have developed evaluation methods for both conjugation and targeting efficiencies that rely on particle characterization techniques, especially the Beckman Coulter Multisizer.  This webinar will present the immunoblot assay (IBA) that measures IgG concentrations in the presence of liposomes.  We use the Multisizer to enumerate population based on size. We will present several sophisticated methods developed by us for determining targeting efficiency (TE), based on the CE and ELISA-measured antibody (Ab) binding affinity and binding efficiency.  The most informative measure of TE, however, is the specific TE, which is the product of the number of Ab molecules that actually bind to immobilized target molecules (determined by comparing the ELISA CE to the IBA CE) and the affinity.

Learning Objectives:
  • Learn about the immunoblot assay (IBA) that measures immunoglobulin G (IgG) concentrations in the presence of liposomes.
  • Learn about sophisticated methods developed for determining targeting efficiency (TE), based on the CE and ELISA-measured antibody (Ab) binding affinity and binding efficiency 

Show Resources
You May Also Like
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
C.E. CREDITS
Date: October 25, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) Candida auris is a multi-drug resistant yeast that continues to be a global threat for infection and transmission in h...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
JUN 29, 2022 8:00 AM PDT
JUN 29, 2022 8:00 AM PDT
Date: June 29, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) As of May 2022, there have been more than 500 million confirmed cases of COVID-19, including numerous variants. Additiona...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
Loading Comments...
Show Resources