OCT 07, 2014 8:00 AM PDT

Comprehensive characterization of mAbs in a single analysis

Sponsored by: Sciex, Sciex
Speaker

Abstract

 

In this Webinar, we will present a comprehensive characterization of a representative monoclonal antibody trypsin-digest in a single analysis. Both small and large peptides (3-65 amino acids) are separated, identified, and quantified, contributing to 100% sequence coverage. Special emphasis is given to comprehensive, quantitative analysis of glycosylation microheterogeneity. Quantitative analysis of degradative hotspots such as asparagine deamidation, methionine oxidation, glutamic acid cyclization, and C-terminal lysine heterogeneity was also achieved from the same run.
The results illustrate the benefits of CESI - the integration of CE and electrospray ionization (ESI) into a single dynamic process within the same device - with high resolution mass spectrometry. The ultra-low flow rate of the system (~20 nL/min) ensures maximal ionization efficiency and dramatically reduces ion suppression.

In this webinar you will learn about:

Obtaining comprehensive heterogeneity, stability, and purity information from a single-enzyme digest in a single run
How ultra-low flow rates reduce ion-suppression bias, increase sensitivity, and expand dynamic range.
Orthogonal technology to status quo


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