OCT 07, 2014 8:00 AM PDT

Comprehensive characterization of mAbs in a single analysis

Sponsored by: Sciex, Sciex
Speaker
  • Applications Scientist, SCIEX Separations
    Biography
      Bryan Fonslow received his Ph.D. in analytical chemistry from the University of Minnesota where he worked with Professor Michael T. Bowser developing micro free-flow electrophoresis for bioanalytical separations and assays. His interest in applying mass spectrometry to bioanalytical separations led him to a postdoctoral position in the laboratory of John R. Yates III at The Scripps Research Institute (TSRI). He is now an application scientist with SCIEX separations and a professional research collaborator at TSRI. His current work focuses on applying capillary electrophoresis separations and mass spectrometry to the analysis of proteins and post-translational modifications.

    Abstract

     

    In this Webinar, we will present a comprehensive characterization of a representative monoclonal antibody trypsin-digest in a single analysis. Both small and large peptides (3-65 amino acids) are separated, identified, and quantified, contributing to 100% sequence coverage. Special emphasis is given to comprehensive, quantitative analysis of glycosylation microheterogeneity. Quantitative analysis of degradative hotspots such as asparagine deamidation, methionine oxidation, glutamic acid cyclization, and C-terminal lysine heterogeneity was also achieved from the same run.
    The results illustrate the benefits of CESI - the integration of CE and electrospray ionization (ESI) into a single dynamic process within the same device - with high resolution mass spectrometry. The ultra-low flow rate of the system (~20 nL/min) ensures maximal ionization efficiency and dramatically reduces ion suppression.

    In this webinar you will learn about:

    Obtaining comprehensive heterogeneity, stability, and purity information from a single-enzyme digest in a single run
    How ultra-low flow rates reduce ion-suppression bias, increase sensitivity, and expand dynamic range.
    Orthogonal technology to status quo


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