APR 11, 2018 9:00 AM PDT

Conversion of Group A Streptococcus Confirmation Testing from Culture to Molecular: Automation, Workflow Improvements and Antimicrobial Stewardship

Sponsored by: DiaSorin Inc.
Speaker
  • Division Director for "Molecular and Microbial Diagnostics and Development", Department of Laboratory Medicine, Geisinger
    Biography
      Dr. Wolk serves as the Division Director for “Molecular and Microbial Diagnostics and Development” in the Department of Laboratory Medicine at Geisinger, an integrated healthcare delivery network serving ~ 3 million people in Central PA.                                                                            A Diplomate of the American Board of Medical Microbiology (ABMM), Dr. Wolk’s professional practice extends to the national and international level. She serves on CLIAC, providing scientific and technical guidance to the US Dept. of Health and Human Services, and serves as a liaison to the CDC’s Board of Scientific Counselors for the Office of Infectious Diseases. She is the Diagnostic Microbiology Lead for Project Santa Fe’s Clinical Laboratory 2.0 initiatives and was recently elected as incoming chair of the Infectious Disease subdivision for the Association of Molecular Pathology. Dr. Wolk is the lead investigator for Geisinger’s Center for Laboratory Innovations and Outcomes, and chair of the Diagnostic Medicine Institute's Clinical Research Council. Her translational research efforts focus on evaluation and evidence-based integration of new technology for infectious disease diagnostics, and she leads the ASM/Center for Disease Control’s Laboratory Medicine Best Practice initiatives to characterize the rapid diagnosis of bacteremia, and is a Professor at Geisinger Commonwealth School of Medicine. In 2012, she was awarded the 1885 Society’s Distinguished Scholar’s Award at the University of Arizona; in 2016 she received the American Society for Microbiology’s BD Award for Clinical Research in Microbiology; in 2018, her career was highlighted in the ASM Press book entitled “Women in Microbiology.” Dr. Wolk’s post-doctoral fellowship was in Medical and Public Health Microbiology at Mayo Clinic, her Ph.D. was awarded in Pathobiology at the University of Arizona, and she earned a Master’s in Health Administration from Wilkes University, with a B.S. in Microbiology/ Medical Technology from Penn State University.

    Abstract

    Streptococcus pyogenes or Group A Streptococcus (GAS) is a pathogenic bacteria that causes both invasive and non-invasive infections resulting in mild to severe life-threatening disease.

    Laboratory guidelines mandate that a more sensitive confirmatory test is performed on negative rapid antigen samples. However, routine culture detection of the organism can take an additional 24-48 hours. As an alternative, rapid molecular testing for Group A Strep can offer a faster and more efficient method for confirmation, contributing to the accelerated treatment of the patient.

    During this presentation you will learn how our laboratory implemented a rapid, automated highthroughput method for molecular confirmation of rapid antigen negatives. The advantages of moving this assay from culture to molecular in terms of workflow and increased sensitivity will be discussed.


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