CRISPR-Based Diagnostics of Kidney Disease

CRISPR-based diagnostics offer rapid and highly sensitive detection of disease biomarkers. Their ease of use and compatibility with lateral-flow readouts make them suitable for point-of-care applications. In this presentation, we introduce technologies that facilitate the read-out of CRISPR/Cas reactions using nanozymes. Specifically, we present CrisprZyme, which utilizes catalytic metallic nanoparticles to enable Cas13-mediated collateral cleavage of reporter molecules. We also highlight their applications in the detection of disease, including biomarkers relevant to transplantation medicine, heart disease, and cancer.

Learning Objectives: 

1. Discuss the advantages and disadvantages of point-of-care diagnostics that are presently employed in clinical routines.

2. Describe the essential features of CRISPR-based diagnostics.

3. Generate innovative ideas for the application of CRISPR-based diagnostics in disease detection.