CRISPR-Cas assisted genetics in intestinal organoids

DATE:  June 24, 2020

TIME:   8:00am PT

The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D intestinal organoid culture system from mouse and human tissues. This novel culture system faithfully recapitulates various aspects of the intestinal epithelium in vitro with remarkable long-term expansion capacity and genetic stability. Thus, the model is recognized as a suitable in vitro model system for genetic studies. To exploit all the potential of this culture, protocols have been fully optimized for primary establishment, maintenance, cryopreservation, plasmid transfection and viral transduction. A number of examples will be shown to introduce how to apply CRISPR technology and organoid models for genetic studies, including simultaneous paralogue knockout, functional genetic screening and precise gene correction.

Learning Objectives:

• Discuss adult stem cell-derived 3D organoid system
• Familiarize yourself with all available genetic tools for the organoid system

Webinars will be available for unlimited on-demand viewing after live event.

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.