JUL 21, 2017 10:00 AM PDT

WEBINAR: CRISPR/Cas9: Moving from Founder Mice to Phenotyping

Speaker
  • Senior Director, Genetic Engineering, Transgenic Genotyping, and Reproductive Sciences , The Jackson Laboratory
    Biography
      Dr. Grass has been involved in the generation of genetically modified mice for more than 25 years. He received his Ph.D. in Molecular Biology from Columbia University in New York, and was a post doctoral fellow at The Sloan Kettering Institute for Cancer Research, where the focus of his research was molecular immunology. Dr. Grass spent an extensive amount of time at a small biotech company (Xenogen Biosciences, previously known as DNX), where his groups were responsible for genetically modified model generation as well as phenotyping and in vivo compound assessment. Dr. Grass is currently the Senior Director, Genetic Engineering, Transgenic Genotyping, and Reproductive Sciences, with responsibility for the model generation program at the Jackson Laboratory.

    Abstract

    DATE: July 21, 2017
    TIME: 10:00am PT, 1:00pm ET

    CRISPR/Cas9 techniques are quickly growing in popularity for the generation of new mouse models for research. Though the approach is relatively simple, many researchers struggle with understanding the complexities associated with the diverse alleles that can be generated, how to deal with mosaicism, and how to move from a single founder mouse to larger cohorts for phenotype characterization.

    Learning Objectives:

    • Understanding the molecular mechanisms that drive the array of possible mutations that can result from a single Cas9/sgRNA targeting strategy
    • A summary of various common targeting strategies to generate indel, knockout, and knockin mutations using CRISPR/Cas9
    • Selection of founder mice and breeding strategies for mitigating mosaicism in founder mice
    • Breeding strategies to establish a colony and generate cohorts for experimentation

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