DATE: May 3, 2017
TIME: 10:00am PT, 1:00pm ET
A number of new instruments have appeared in recent times to accommodate different applications and highlight technology advances. This webinar will focus on advances in the Invitrogen™ Attune™ NxT Flow Cytometer and outline where the technology within the Attune NxT fits in current and future applications.
I will discuss how the core acoustic focusing technology in the instrument was developed and how it has evolved. Many flow cytometers have unique capabilities and the Attune NxT is no exception. For example, the combination of traditional hydrodynamic focusing in addition to acoustic focusing allows the Attune NxT to achieve some unique tasks such as very high sample flow rates with exceptional CVs. Everyone knows that flow cytometry offers extreme capability with single cell analysis, but we also know how difficult it is to run very small but valuable samples. A unique feature of the Attune NxT is that you can dilute that specimen by orders of magnitude and still run the sample in a reasonable time because the sample flow rate can be drastically increased without altering the quality of the data. This feature alone can be responsible for saving critical samples that normally may be lost. In this regard it is pertinent to understand how and why the Attune NxT fluidics is capable of operating under these conditions and I will explain the fundamentals of the how the high sample flow technology actually works. While discussing technology, I will also explain how the Attune NxT uses a special Coherent designed laser-optics module delivery system to achieve a unique flattop (tophat) focused illumination spot that plays a significant role in the low CVs achieved under high sample flow. I will also discuss my experience with several other aspects of the core technology in the Attune NxT and show how its automated plate reader option can enhance unassisted throughput giving operators back valuable time.
For Research Use only. Not for use in diagnostic procedures.
- Compare hydrodynamic and acoustic focusing in flow cytometry
- List three applications where the Attune NxT provides a unique benefit
- Describe three automated plate reader options for the Attune NxT