MENU

Deciphering the spreading of neuropathologies in neuronal circuits using a high capacity microfluidics platform

Presented at: Neuroscience 2020
Speaker

Abstract

Misfolded and accumulated neurodegenerative disease associated proteins (NDAPs, such as tau and alpha-synuclein) represent the major pathological hallmark in Alzheimer’s and Parkinson’s disease patient brain cells. Compelling evidence indicate that spreading of NDAPs within the patient brain occurs via interconnected neurons. While progress in deciphering the mechanisms of neuronal uptake of NDAPs and NDAP-caused protein aggregation has been made, our knowledge about other cellular processes, such as axonal uptake and transport of NDAPs as well as neuron-to-neuron transfer of NDAPs, is still limited. In the presentation, I will present Cellectricon’s approach to decipher individual cellular processes that are occurring in progressive proteopathies in cortical neuronal circuits. We apply an experimental in vitro paradigm to generate compartmentalized neuronal cells by culturing primary mouse cortical neurons in high capacity microfluidic co-culture plates. By using fluorescently labelled, seed competent NDAPs and high-content imaging, we can assess NDAP axonal uptake, axonal transport and protein aggregation in cortical neurons. By combining our neuronal compartmentalisation approach with neuronal labelling techniques, we aim to decipher the spreading mechanisms of neuropathologies in neuronal circuits. We aim to provide insights into specific disease-relevant neuronal processes, which can pave the way for novel intervention approaches for modulation of progressive proteopathies.

Learning Objectives:

1. Introduce the audience the principles of progressive neurodegenerative diseases

2. Present a concept that enables the assessment of specific cellular processes involved in progressive neurodegenerative diseases

3. Present data obtained from Cellectricon´s high-capacity microfluidics platform showing alpha-synuclein axonal uptake, transport and caused protein aggregation in a sub-population of primary cortical neurons


Show Resources
You May Also Like
JUN 28, 2022 6:45 AM PDT
JUN 28, 2022 6:45 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
Loading Comments...
Show Resources