MAY 14, 2015 10:30 AM PDT

Deep mutational scanning to measure the impact of mutations in proteins on the molecular and organismal level

Speaker

Abstract

Deep mutational scanning is a method that marries selection for protein function amongst a large library of protein variants with high-throughput DNA sequencing to measure the activity of hundreds of thousands of variants simultaneously. The result is a sequence-function map that describes the impact of all possible single and many double mutants on protein function. We have shown that sequence-function maps have many uses. For example, we are analyzing them to learn about protein properties like structure, aggregation, stability and enzyme mechanism; to guide the interpretation of coding variants in genomes; and to better understand protein evolution. 1. Describe progress in computational and experimental methods for interpreting the consequences of variation in genomes. 2. Evaluate the strengths and weaknesses of different approaches for interpreting variation.


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MAY 14, 2015 10:30 AM PDT

Deep mutational scanning to measure the impact of mutations in proteins on the molecular and organismal level


Specialty

Research And Development

Gene Expression

Big Data

Dna

Cancer Research

Tumor

Biomarkers

Cancer

Earth Science

Oncology

University

Gene Sequencing

Mass Cytometry

Drug Discovery

Cell Culture

Geography

Asia50%

Europe50%

Registration Source

Website Visitors100%

Job Title

Student50%

Medical Laboratory Technician50%

Organization

Manufacturer - Other50%

Academic Institution50%


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